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Thorlabs Imaging Systems


Thorlabs Imaging Systems


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Purpose-Built Imaging Platforms

Thorlabs' microscopy systems combine the full benefits of a vertically integrated organization with decades of expertise to tailor virtually every component incorporated into its platforms. Our scientists and engineers draw from experience in all key disciplines to design, develop, manufacture, and support advanced imaging systems.

Our systems are designed from a clean sheet of paper to do the job intended from the beginning. Leveraging our extensive in-house design and fabrication capabilities allows us to minimize design trade-offs, develop targeted solutions to challenging problems, and customize each individual system to our customers' specifications.

 

Bergamo II Microscope
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Rotating Bergamo II Microscopes Offer 100° of Focal Plane Rotation

 

Bergamo® II Series Multiphoton Microscopes

Recognizing that there is a need for a microscope with a modular structure that can be configured to the experiment, rather than the other way around, we set out to develop the new reference standard in multiphoton microscopy. With a selection of rigid bodies, numerous auxiliary imaging modalities, detectors, motion control, and laser beam control, the permutations of the Bergamo II Series are endless.

For additional information, please see the full Bergamo II Series presentation.

 

EnVista Whole-Slide-Scanning Microscope
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TIDE™ Whole-Slide-Scanning Research Microscope Configured for Brightfield and Fluorescence

 

TIDE™ Whole-Slide-Scanning Research Microscope

Our TIDE™ whole-slide imaging system is a fast, accurate solution built on a research microscope. Available for brightfield and fluorescence microscopy, TIDE offers greatly increased imaging speeds (up to 5 times) when compared to stop-and-stare techniques, as well as large-format images with reduced alignment errors that do not require stitching.

For additional information, please see the full TIDE presentation.

 

Bergamo II Microscope
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The Multiphoton Mesoscope Utilizes Technology Under License with HHMI's Janelia Research Campus

 

Multiphoton Mesoscope

To analyze large sections of the brain operating in concert, we developed a two-photon mesoscope for rapid functional imaging over a large Ø5 mm field of view (FOV). Scan over the entire FOV or rapidly image multiple spatially separated regions. The mesoscope enables in vivo awake-specimen neural studies at sub-cellular resolution with near-video imaging frame rates.

For additional information, please see the full Multiphoton Mesoscope presentation.

 

Cerna
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Cerna Rig Configured for Single-Cell Recordings

 

Cerna® Series Widefield Imaging and
Electrophysiology Microscopes

The Cerna Series is Thorlabs' platform for electrophysiology, optogenetics, DNA injection, and other applications that require widefield and transmitted light imaging. Its versatile, expandable, accessible design is fully compatible with Thorlabs-manufactured and user-supplied accessories.

For additional information, please see the full Cerna Series presentation.

 

Confocal Microscope
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Inverted Microscope Upgraded with Thorlabs Confocal Scan Head

 

Confocal Microscopy Systems

Thorlabs' Confocal Laser Scanning Microscopy Systems are designed to add confocal imaging capabilities to research-grade microscopes. Leveraging the same 400 FPS resonant scanning technology as its multiphoton cousins and supporting up to four excitation wavelengths and detection channels, it enables multiplexed, multichannel experiments with live cells.

For additional information, please see the full Confocal Microscopy presentation.

 

Confocal Microscope
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Telesto-II Variable-Rate Spectral Domain OCT System

 

Spectral Domain OCT Systems

Thorlabs has developed several spectral domain OCT system families to accommodate the specific needs of each application. Our Telesto, Callisto, and Ganymede systems offer a choice of 930 nm or 1325 nm center wavelengths and are balanced to offer the best possible combination of scan rates, sensitivities, FOV, and axial and lateral resolution.

Please click here for comparisons between spectral domain and swept source OCT systems, or click here to browse our full selection of spectral domain systems.

 

Tiberius Laser
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Femtosecond Ti:Sapphire Laser for Multiphoton Imaging

 

Tunable Femtosecond Ti:Sapphire Laser

Designed in close collaboration with Thorlabs' life science application specialists, the Tiberius® tunable femtosecond laser is ideally suited for two-photon imaging. Manufactured in-house, it offers an industry-leading tuning speed of up to 200 nm in <50 ms and a unique vertical cavity construction that reduces the footprint to about half that of competing designs.

For additional information, please see the full Tiberius presentation.

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please contact ImagingSales@thorlabs.com or call (703) 651-1700.

In Vivo Deep Tissue Imaging

Deep tissue imaging is a hallmark of multiphoton microscopy. The Bergamo® II Series' ultrasensitive detector module utilizes full-field-of-view, non-descanned GaAsP PMT detectors to maximize the collection of photons scattered deep within a sample. This efficient design allows morphological features deep within a sample to be reconstructed without having to excise tissue.

The image to the left shows an XZ projection of the primary somatosensory cortex in an 8-week-old mouse expressing thy1-YFP (H line), with a sample depth in excess of 1.0 mm. The excitation wavelength was 960 nm and the data was taken with a Nikon Apo LWD 25X objective with NA 1.10. The data set is courtesy of Dr. Hajime Hirase and Katsuya Ozawa, RIKEN Brain Science Institute, Wako, Japan.

 

EnVista Whole Slide Image
Click Here for Enlarged View of Whole-Slide Image
Click Here for Enlarged View of Inset
Whole-Slide and Detail Image of Bovine Pulmonary Artery Endothelial (BPAE) Cells 

Whole-Slide Fluorescence Image

A fluorescence image of bovine pulmonary artery endothelial (BPAE) cells. The mitochondria were stained with MitoTracker® Red CMXRos, the f-actin was stained with Alexa Fluor® 488 phalloidin, and the nuclei were counterstained with DAPI. The image (scan area 15 mm x 15 mm) was taken at 20X magnification (caluciated using the DICOM standard) The inset shows a 604 µm x 627 µm area of the whole-slide view.

This image was taken with Thorlabs' TIDE™ Whole-Slide-Scanning System. The excitation wavelength was 1300 nm.

 

Developmental Biology

Maximum projection (dorsal and lateral, respectively) of zebrafish embryos, 48 hrs post-fertilization, showing developing neurons. Neurons are labeled with Alexa 488 against acetylated tubulin.

These images were taken using Thorlabs' Confocal Microscope. The fluorophore was excited using a 488 nm laser and imaged with an Olympus 20X 1.0 NA water dipping lens. The data set is courtesy of Aminah Giousoh and Dr. Robert Bryson-Richardson, School of Biological Sciences, Monash University, Victoria, Australia.

 

Merged Image
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Merged Image

Structural Insight with Nonlinear Imaging

Although fluorescent proteins can overcome the limitations of thick or in vivo samples, where it is not always easy for fluorophores to penetrate, their use is not always desired. Nonlinear microscopy, of which two-photon fluorescence is a part, offers several complementary contrast mechanisms for visualizing structure. Coherent Anti-Stokes Raman Scattering (CARS), Second Harmonic Generation (two-photon fluorescence), and Third Harmonic Generation (three-photon fluorescence) are nonlinear imaging techniques that offer contrast that is the direct result of the interaction of the laser with the structure of interest. Under specific conditions, this allows the generation of an observable signal without the need for fluorophores.

The figure to the right shows an image made by combining CARS, SHG, and Sum Frequency Generation (SFG) signals, while the figures below shows the individual signals from each of these imaging modalities. These images were taken using a Thorlabs multiphoton microscope.

 

High-Resolution Imaging
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Dendritic Spines Expressing tdTomato

High-Resolution Imaging

Expanded view of dendritic spines from neurons in the mouse visual cortex expressing tdTomato.

In this image, taken with a Thorlabs multiphoton microscope, the excitation wavelength was 1040 nm, and an Olympus 60X objective with NA 1.0 was used. This image is courtesy of Dr. Tobias Rose, Max Planck Institute for Neurobiology, Martinsreid, Germany.

 

OCT Scan Control Screen Shot
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Image of a Wasp Cadaver in Volume Rendering Mode

Deep 3D Volumetric Imaging

An image of a wasp cadaver. The optical video image of the sample can be seen in the bottom right corner.

This image was taken with Thorlabs' TELESTO-II Spectral Domain OCT System. The excitation wavelength was 1300 nm.

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please contact ImagingSales@thorlabs.com or call (703) 651-1700.

Deep Insights

 

In Vivo Functional Neural Imaging of Large Volumes

Large FOV imaging at high spatiotemporal resolution is a necessary requirement for correlating neural activity of large brain volumes. This movie demonstrates the firing of neurons expressing GCaMP6f in behaving mice running on a spherical treadmill. The low magnification movie shows activity across multiple brain areas, including the somatosensory, parietal, and motor areas, at 4.3 Hz across a 6.3 mm x 5.4 mm FOV. The four non-contiguous regions were imaged at 9.6 Hz with 600 μm x 600 μm FOV, and fluorescence signals from individual neurons were sampled to cross-correlate their activity.

In these image sets, taken with the Multiphoton Mesoscope, the excitation wavelength was 970 nm. The data set is courtesy of Nicholas James Sofroniew, Daniel Flickinger, Jonathan King, and Karel Svoboda; Janelia Research Campus and Vidrio Technologies, Virginia, USA (video taken from https://elifesciences.org/content/5/e14472/article-data and used here under a Creative Commons Attribution license).

 

Intravital Imaging

The ability to image regions within an organ of a living animal (and not just very thin tissue, like mesentery tissue) represents a major advancement in intravital microscopy. In this movie, blood perfusion can be monitored. Blood is labeled with a tail vein injection of FITC-Dextran containing red fluorescent microspheres. The microspheres can be tracked to indicate blood velocity. Red blood cells are also visible as dark shadows within the green vessels.

In these images, taken with a Thorlabs multiphoton microscope, the excitation wavelength was 780 nm and an Olympus XLUMPLFLN 20X objective with NA 1.0 was used. The preparation is courtesy of Dr. Simon Rhodes, IUPUI School of Medicine, Indianapolis, Indiana.

Deep Brain Imaging

Deep tissue imaging is a hallmark of multiphoton microscopy. The Bergamo® II Series' ultrasensitive detector module utilizes full-field-of-view, non-descanned GaAsP PMT detectors to maximize the collection of photons scattered deep within a sample. This efficient design allows morphological features deep within a sample to be reconstructed without having to excise tissue.

The images shown above represent an XZ projection of the primary somatosensory cortex in an 8-week-old mouse expressing thy1-YFP (H line), with a sample depth in excess of 1.0 mm. The excitation wavelength was 960 nm and the data was taken with a Nikon Apo LWD 25X objective with NA 1.10. The data set is courtesy of Dr. Hajime Hirase and Katsuya Ozawa, RIKEN Brain Science Institute, Wako, Japan.

 

Confocal Reflectance Microscopy

Not all samples can be labeled with an exogenous contrast agent. Confocal reflectance microscopy allows for optical sectioning imaging by observing reflected laser light. This movie represents a Z-stack of a peachworm.

These images were taken with our Confocal Microscope. The excitation wavelength was 488 nm and the data was taken with an Olympus UPlanFl 20X objective with NA 0.50.

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please contact ImagingSales@thorlabs.com or call (703) 651-1700.

Thorlabs' sales engineers and field service staff are based out of eight offices across four continents. We look forward to helping you determine the best imaging system to meet your specific experimental needs. Our customers are attempting to solve biology's most important problems; these endeavors require matching systems that drive industry standards for ease of use, reliability, and raw capability.

Thorlabs' worldwide network allows us to operate showrooms in a number of locations where you can see our systems in action. We welcome the opportunity for personal interaction during your visit! A demo can be scheduled at any of our showrooms by contacting ImagingSales@thorlabs.com.

Showroom Icon

Showroom and Customer Support Sites

 

Sterling, Virginia, USA
Thorlabs Imaging Systems HQ

Thorlabs Imaging Systems
108 Powers Court
Sterling, VA 20166

Appointment Scheduling and Customer Support

 

China

Thorlabs China
Room A101, No. 100, Lane 2891, South Qilianshan Road
Shanghai 200331

Appointment Scheduling and Customer Support

 

Japan

Thorlabs Japan, Inc.
Higashi-Ikebukuro Q Building, 2-23-2
Higashi-Ikebukuro, Toshima-ku, Tokyo 170-0013

Appointment Scheduling and Customer Support

Customer Support Sites

 

Newton, New Jersey, USA
Thorlabs HQ

Thorlabs, Inc.
56 Sparta Avenue
Newton, NJ 07860

Customer Support

 

United Kingdom

Thorlabs Ltd.
1 Saint Thomas Place, Ely
Ely CB7 4EX

Customer Support

 

Germany

Thorlabs GmbH
Hans-Boeckler-Str. 6
Dachau/Munich 85221

Customer Support

 

France

Thorlabs SAS
109, rue des Cotes
Maisons-Laffitte 78600

Customer Support

 

Brazil

Thorlabs Vendas de Fotônicos Ltda.
Rua Riachuelo, 171
São Carlos, SP 13560-110

Customer Support

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please contact ImagingSales@thorlabs.com or call (703) 651-1700.


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Last Edited: Nov 08, 2013 Author: Dan Daranciang