Fluorescence Imaging Filters

Excitation, Emission, and Dichroic Filters for Imaging Applications
Matched Filter Sets Designed for Wavelength Ranges of Common Fluorophores
Compatible with Filter Cubes for 12.5 mm, 25 mm, or 32 mm Filters

MDF05-BFP

12.5 mm Filter Set for Blue Fluorescent Protein

Exploded View of a 25 mm MDF-GFP Filter Set Installed Into a TLV-TE2000 Filter Cube

Dichroic Mirror Inside Cube

Excitation
Filter

Emission
Filter

Image Plane

Retaining
Ring

MDF-CY5

25 mm Filter Set for Cy5

MDF32-NIR

32 mm Filter Set for LICOR IRDye 800CW

Please Wait

Overview
Specs
Tutorial
Feedback

Figure 1.2 Dichroic filters have (a) an engraving on or (b) a carat indicating the primary reflective surface. Light should be incident on this side for best performance.

Figure 1.1
Excitation and emission filters are marked with an arrow that shows the recommended direction of light propagation.

Features

Excitation, Emission, and Dichroic Filters for Fluorescence Imaging
>90% Transmission over Desired Wavelength Band
Sharp Cutoff (Up to T < 0.001%) Outside the Transmission Band
Matched Filter Sets Designed to Target Many Common Fluorophores

- BFP
- CFP
- WGFP
- GFP
- FITC
- Alexa Fluor^®∗ 488 - YFP
- tdTomato
- TRITC
- Texas Red^®*
- mCherry
- Cy^®†3.5 - Cy5
- Cy5.5
- Cy7
- LI-COR IRDye^®‡ 800CW
Excitation and Emission Filters are Mounted in Black-Anodized Aluminium Housings
Dichroic Filters are Unmounted
Compatible with Filter Cubes Designed for 12.5 mm, 25 mm, or 32 mm Optics
Filter Sets are Available at a Savings Over Individual Filter Prices

Filter Options
12.5 mm Filter Sets
25 mm Filter Sets
Ø25 mm Excitation Filters
Ø25 mm Emission Filters
25 mm x 36.0 mm Dichroic Filters
32 mm Filter Sets

These excitation, emission, and dichroic filters are designed specifically for use in fluorescence imaging applications. They are fabricated at industry-standard dimensions that make them compatible with filter cubes from all major manufacturers or with our cage cubes. We offer filters designed to target the following common fluorophores: BFP, CFP, WGFP, GFP, FITC, Alexa Fluor 488, YFP, tdTomato, TRITC, Texas Red, mCherry, Cy3.5, Cy5, Cy5.5, Cy7, and LI-COR IRDye 800CW. While many of the filters are offered individually, some are only offered in a three-piece set containing the excitation, emission, and dichroic filters; see the Specs tab for details. A selection of these filters are also available pre-installed into our microscope filter cubes.

Filter Design
Our filters are manufactured to high-performance optical specifications and designed for durability. They are produced via multiple dielectric layers deposited on a high-precision, fused silica substrate. The substrate is ground and polished to ensure that the highest possible image quality is maintained. The resulting hard-coated optics consist of filter layers that are denser than those obtained from electron beam deposition techniques, and which reduce water absorption while greatly enhancing durability, stability, and performance of the filter. Each filter layer is monitored during growth to ensure minimal deviation from design specification thickness, ensuring overall high-quality filter performance. Each excitation or emission filter is housed in a Ø12.5 mm, Ø25 mm, or Ø32 mm black anodized aluminum ring which makes handling easier and enhances the blocking OD by limiting scattering.

Click to Enlarge
Figure 1.3 The 32 mm MDF32-TOM filter set being installed in a DFM32T1 cage cube insert.

Compatibility
Thorlabs offers mounting solutions for the products sold here. The Ø12.5 mm filter sets are compatible with our 16 mm Cage Cubes for 12.5 mm filters while the Ø25 mm filters and filter sets are compatible with our 30 mm Cage Cubes for 25 mm filters. For customers who wish to use the Ø25 mm filters in Thorlabs, Olympus, or Nikon fluorescence microscopes, Thorlabs manufactures a family of Drop-In Microscope Filter Cubes. Finally, the Ø32 mm filter sets are compatible with our 30 mm Cage Cubes for 32 mm filters.

The excitation and emission filters can be mounted in our extensive line of filter mounts and wheels. As their aluminum rings are not threaded, compatible retaining rings will be required to mount the filters in one of our internally-threaded lens tubes. Additionally, the unmounted dichroic filters can be mounted in a compatible rectangular kinematic mount, which are designed to secure a 1 mm thick rectangular optic with minimal stress.

^∗Alexa Fluor and Texas Red are registered trademarks of Molecular Probes, Inc.
^†Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH
^‡IRDye is a registered trademark of LI-COR Biotech, LLC

Fluorophore	Filter Size	Set Item #	Filter Item #	Filter Type	Center Wavelength	FWHM	Reflection Band	Transmission Band
BFP	12.5 mm	MDF05-BFP	-	Excitation	395 nm	25 nm	-	-
			-	Emission	460 nm	49 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 350 - 418 nm	T_avg ≥ 95% 433 - 750 nm
	25 mm	MDF-BFP	MF390-18	Excitation	390 nm	18 nm	-	-
			MF460-60	Emission	460 nm	60 nm	-	-
			MD416^b	Dichroic	-	-	R_avg > 90% 360 - 407 nm	T_abs > 90% 425 - 575 nm
	32 mm	MDF32-BFP	-	Excitation	395 nm	25 nm	-	-
			-	Emission	460 nm	49 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 350 - 418 nm	T_avg ≥ 95% 433 - 750 nm
CFP	25 mm	MDF-CFP	MF434-17	Excitation	434 nm	17 nm	-	-
			MF479-40	Emission	479 nm	40 nm	-	-
			MD453^b	Dichroic	-	-	R_avg > 90% 423 - 445 nm	T_abs > 90% 460 - 610 nm
WGFP	25 mm	MDF-WGFP	MF445-45	Excitation	445 nm	45 nm	-	-
			MF510-42	Emission	510 nm	42 nm	-	-
			MD480^b	Dichroic	-	-	R_avg > 90% 415 - 470 nm	T_abs > 90% 490 - 720 nm
GFP	12.5 mm	MDF05-GFP	-	Excitation	469.5 nm	40 nm	-	-
			-	Emission	524.5 nm	48 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 442 - 488 nm	T_avg ≥ 95% 502 - 552 nm
	25 mm	MDF-GFP	MF469-35	Excitation	469 nm	35 nm	-	-
			MF525-39	Emission	525 nm	39 nm	-	-
			MD498^b	Dichroic	-	-	R_avg ≥ 90% 452 - 490 nm	T_abs ≥ 90% 505 - 800 nm
	32 mm	MDF32-GFP	-	Excitation	469.5 nm	40 nm	-	-
			-	Emission	524.5 nm	48 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 442 - 488 nm	T_avg ≥ 95% 502 - 552 nm
FITC	12.5 mm	MDF05-FITC	-	Excitation	479 nm	30 nm	-	-
			-	Emission	537 nm	40 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 94% 380 - 497 nm	T_avg ≥ 92% 514.5 - 900 nm
	25 mm	MDF-FITC	MF475-35	Excitation	475 nm	35 nm	-	-
			MF530-43	Emission	530 nm	43 nm	-	-
			MD499^b	Dichroic	-	-	R_avg > 90% 470 - 490 nm	T_abs > 90% 508 - 675 nm
	32 mm	MDF32-FITC	-	Excitation	479 nm	30 nm	-	-
			-	Emission	537 nm	40 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 94% 380 - 497 nm	T_avg ≥ 92% 514.5 - 900 nm
GFP Alexa Fluor^® 488	25 mm	MDF-GFP2	-	Excitation	482 nm	18 nm	-	-
			-	Emission	520 nm	28 nm	-	-
			-	Dichroic	-	-	R_avg > 93% 350 - 488 nm	T_avg > 93% 502 - 950 nm
YFP	25 mm	MDF-YFP	MF497-16	Excitation	497 nm	16 nm	-	-
			MF535-22	Emission	535 nm	22 nm	-	-
			MD515	Dichroic	-	-	R_avg > 90% 490 - 510 nm	T_abs > 90% 525 - 700 nm
tdTomato	12.5 mm	MDF05-TOM	-	Excitation	540 nm	25 nm	-	-
			-	Emission	605 nm	55 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 94% 450 - 556.5 nm	T_avg ≥ 92% 575 - 900 nm
	25 mm	MDF-TOM	-	Excitation	531 nm	40 nm	-	-
			-	Emission	593 nm	40 nm	-	-
			-	Dichroic	-	-	R_avg > 98% 350 - 555 nm	T_avg > 93% 569 - 950 nm
	32 mm	MDF32-TOM	-	Excitation	540 nm	25 nm	-	-
			-	Emission	605 nm	55 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 94% 450 - 556.5 nm	T_avg ≥ 92% 575 - 900 nm
TRITC	25 mm	MDF-TRITC	MF542-20	Excitation	542 nm	20 nm	-	-
			MF620-52^c	Emission	620 nm	52 nm	-	-
			MD568^b	Dichroic	-	-	R_avg > 90% 525 - 556 nm	T_abs > 90% 580 - 650 nm
Texas Red	12.5 mm	MDF05-TXRED	-	Excitation	562 nm	42 nm	-	-
			-	Emission	635.5 nm	58 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 94% 460 - 587 nm	T_avg ≥ 92% 608 - 900 nm
	25 mm	MDF-TXRED	MF559-34	Excitation	559 nm	34 nm	-	-
			MF630-69	Emission	630 nm	69 nm	-	-
			MD588^b,d	Dichroic	-	-	R_avg > 90% 533 - 580 nm	T_abs > 90% 595 - 800 nm
	32 mm	MDF32-TXRED	-	Excitation	562 nm	42 nm	-	-
			-	Emission	635.5 nm	58 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 94% 460 - 587 nm	T_avg ≥ 92% 608 - 900 nm
mCherry	12.5 mm	MDF05-MCHC	-	Excitation	560 nm	40 nm	-	-
			-	Emission	629 nm	71 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 450 - 578 nm	T_avg ≥ 95% 593 - 700 nm
	25 mm	MDF-MCHC	-	Excitation	562 nm	40 nm	-	-
			-	Emission	641 nm	75 nm	-	-
			-	Dichroic	-	-	R_avg > 98% 350 - 585 nm	T_avg > 93% 601 - 950 nm
	32 mm	MDF32-MCHC	-	Excitation	560 nm	40 nm	-	-
			-	Emission	629 nm	71 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 450 - 578 nm	T_avg ≥ 95% 593 - 700 nm
Cy3.5	25 mm	MDF-CY3.5	MF565-24	Excitation	565 nm	24 nm	-	-
			MF620-52^c	Emission	620 nm	52 nm	-	-
			MD588^b,d	Dichroic	-	-	R_avg > 90% 533 - 580 nm	T_abs > 90% 595 - 800 nm
mCherry (578 nm Excitation)	25 mm	MDF-MCHA	-	Excitation	578 nm	21 nm	-	-
			-	Emission	641 nm	75 nm	-	-
			-	Dichroic	-	-	R_avg > 98% 350 - 588 nm	T_avg > 93% 603 - 950 nm
Cy5	12.5 mm	MDF05-CY5	-	Excitation	619 nm	60 nm	-	-
			-	Emission	700 nm	71 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 500 - 648 nm	T_avg ≥ 95% 666 - 890 nm
	25 mm	MDF-CY5	-	Excitation	619 nm	60 nm	-	-
			-	Emission	700 nm	71 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 500 - 648 nm	T_avg ≥ 95% 666 - 890 nm
	32 mm	MDF32-CY5	-	Excitation	619 nm	60 nm	-	-
			-	Emission	700 nm	71 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 500 - 648 nm	T_avg ≥ 95% 666 - 890 nm
Cy5.5	12.5 mm	MDF05-CY55	-	Excitation	650 nm	44 nm	-	-
			-	Emission	720 nm	59.5 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 520 - 674 nm	T_avg ≥ 95% 692 - 1000 nm
	25 mm	MDF-CY55	-	Excitation	650 nm	44 nm	-	-
			-	Emission	720 nm	59.5 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 520 - 674 nm	T_avg ≥ 95% 692 - 1000 nm
	32 mm	MDF32-CY55	-	Excitation	650 nm	44 nm	-	-
			-	Emission	720 nm	59.5 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 520 - 674 nm	T_avg ≥ 95% 692 - 1000 nm
Cy7	12.5 mm	MDF05-CY7	-	Excitation	709 nm	75.5 nm	-	-
			-	Emission	813 nm	96 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 570 - 746 nm	T_avg ≥ 95% 766 - 1100 nm
	25 mm	MDF-CY7	-	Excitation	709 nm	75.5 nm	-	-
			-	Emission	813 nm	96 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 570 - 746 nm	T_avg ≥ 95% 766 - 1100 nm
	32 mm	MDF32-CY7	-	Excitation	709 nm	75.5 nm	-	-
			-	Emission	813 nm	96 nm	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 570 - 746 nm	T_avg ≥ 95% 766 - 1100 nm
IRDye^® 800CW	12.5 mm	MDF05-NIR	-	Excitation	741 nm	41 nm	-	-
			-	Emission	N/A^e	-	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 580 - 760 nm	T_avg ≥ 95% 780 - 1100 nm
	25 mm	MDF-NIR	-	Excitation	741 nm	41 nm	-	-
			-	Emission	N/A^e	-	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 580 - 760 nm	T_avg ≥ 95% 780 - 1100 nm
	32 mm	MDF32-NIR	-	Excitation	741 nm	41 nm	-	-
			-	Emission	N/A^e	-	-	-
			-	Dichroic	-	-	R_avg ≥ 98% 580 - 760 nm	T_avg ≥ 95% 780 - 1100 nm

Click on for a plot and downloadable data.
These dichroics have an AR coating designed for 45° AOI on the back surface with R_abs < 2% from 400 to 800 nm.
Designed to work with both TRITC and CY3.5.
Designed to work with both CY3.5 and TXRED.
This emission filter has a cut on wavelength of 780 nm with average transmission ≥94% for 790 - 1000 nm

Common Specifications for 12.5 mm Filter Sets

Item # Suffix	Size	Clear Aperture	Angle of Incidence	Thickness	Surface Quality	Substrate
Excitation Filters
BFP, GFP, MCHC, CY5, CY55, CY7, NIR	Ø12.5 ± 0.1 mm	≥Ø9.5 mm	0° ± 5°	5.0 ± 0.1 mm	60-40 Scratch-Dig	Alkaline Earth Boro-Aluminosilicate
FITC, TOM, TXRED	80-50 Scratch-Dig
Emission Filters
BFP, GFP, MCHC, CY5, CY55, CY7, NIR	Ø12.5 ± 0.1 mm	≥Ø9.5 mm	0° ± 5°	3.5 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
FITC, TOM, TXRED	80-50 Scratch-Dig	Alkaline Earth Boro-Aluminosilicate
Dichroic Filters
BFP, GFP, MCHC, CY5, CY55, CY7, NIR	12.5 mm x 17.6 mm	≥(10 mm x 15 mm)	45° ± 1.5°	1.0 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
FITC, TOM, TXRED	1.1 +0.15/-0.1 mm	80-50 Scratch-Dig	Alkaline Earth Boro-Aluminosilicate

Common Specifications for 25 mm Filters

Item #	Size	Clear Aperture	Angle of Incidence	Thickness	Surface Quality	Substrate
Excitation Filters
MF390-18, MF434-17, MF445-45, MF469-35, MF475-35, MF497-16, MF542-20, MF559-34, MF565-24	Ø25 ± 0.1 mm	>Ø21 mm	0° ± 5	5.0 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
Emission Filters
MF460-60, MF479-40, MF510-42, MF525-39, MF530-43, MF535-22, MF620-52, MF630-69	Ø25 ± 0.1 mm	>Ø21 mm	0° ± 5°	3.5 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
Dichroic Filters
MD416, MD453, MD480, MD498, MD499, MD515, MD568, MD588	25.0 mm x 36.0 mm	>(22.5 mm x 32.4 mm)	45° ± 1.5°	1.0 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica

Common Specifications for 25 mm Filter Sets

Item # Suffix	Size	Clear Aperture	Angle of Incidence	Thickness	Surface Quality	Substrate
Excitation Filters
GFP2, TOM, MCHC, MCHA	Ø25 +0.0/-0.1 mm	>Ø21 mm	0° ± 5	5.0 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
CY5, CY55, CY7, NIR	Ø25.0 ± 0.1 mm	≥Ø22 mm	Alkaline Earth Boro-Aluminosilicate
BFP, CFP, WGFP, GFP, FITC, YFP, TRITC, TXRED, CY3.5	Ø25 ± 0.1 mm	>Ø21 mm	Fused Silica
Emission Filters
GFP2, TOM, MCHC, MCHA	Ø25 +0.0/-0.1 mm	>Ø22 mm	0° ± 5°	3.5 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
CY5, CY55, CY7, NIR	Ø25.0 ± 0.1 mm	≥Ø22 mm
BFP, CFP, WGFP, GFP, FITC, YFP, TRITC, TXRED, CY3.5	Ø25 ± 0.1 mm	>Ø21 mm
Dichroic Filters
GFP2, TOM, MCHC, MCHA	25.2 mm x 35.6 mm	80% of Area	45° ± 1.5°	1.05 ± 0.05 mm	60-40 Scratch-Dig	Fused Silica
CY5, CY55, CY7, NIR	25.0 mm x 35.8 mm	≥22 mm x 33 mm (Elliptical)	1.0 ± 0.1 mm
BFP, CFP, WGFP, GFP, FITC, YFP, TRITC, TXRED, CY3.5	25.0 mm x 36.0 mm	>(22.5 mm x 32.4 mm)

Common Specifications for 32 mm Filter Sets

Item # Suffix	Size	Clear Aperture	Angle of Incidence	Thickness	Surface Quality	Substrate
Excitation Filters
BFP, GFP, MCHC, CY5, CY55, CY7, NIR	Ø32.0 ± 0.1 mm	≥Ø29.0 mm	0° ± 5°	5.0 ± 0.1 mm	60-40 Scratch-Dig	Alkaline Earth Boro-Aluminosilicate
FITC, TOM, TXRED	80-50 Scratch-Dig
Emission Filters
BFP, GFP, MCHC, CY5, CY55, CY7, NIR	Ø32.0 ± 0.1 mm	≥Ø29.0 mm	0° ± 5°	3.5 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
FITC, TOM, TXRED	80-50 Scratch-Dig	Alkaline Earth Boro-Aluminosilicate
Dichroic Filters
BFP, GFP, MCHC, CY5, CY55, CY7, NIR	32.0 mm x 44.0 mm	≥(30 mm x 42 mm)	45° ± 1.5°	1.0 ± 0.1 mm	60-40 Scratch-Dig	Fused Silica
FITC, TOM, TXRED	1.1 +0.15/-0.1 mm	80-50 Scratch-Dig	Alkaline Earth Boro-Aluminosilicate

Click to Enlarge
Figure 3.1 Schematic of light passing through a fluorescence microscope.

Excitation filter, Emission filter, and dichroic mirror transmission plots

Click to Enlarge
Figure 3.2 MDF-YFP filter set transmission graph. Note the dichroic mirror (green) reflects light in the excitation wavelength range (blue), and transmits light in the emission wavelength range (green).

Filters for Fluorescence Microscopy

Fluorophores
A fluorophore is a molecule or portion of a molecule that is capable of producing fluorescence. When light of the appropriate frequency necessary to excite a molecule from its ground state to an excited state is present, excitation will occur. However, once in an excited state, the molecule will be unstable. After some short period of time (typically 10^-15 to 10^-9 s), a photon will be released, thereby enabling the molecule to return to a lower energy state. The emitted radiation will be at a longer wavelength (lower energy) than the absorbed radiation due to the loss of energy through various mechanisms such as vibrations, sound, and thermal energy.

A single fluorophore can be continually excited unless it is destroyed by photobleaching (i.e. the nonreversible destruction of a fluorophore due to photon-induced chemical damage or covalent modification). The average number of excitation and emission cycles that a particular fluorophore can undergo prior to photobleaching depends on its molecular structure and the local environment; some fluorophores bleach quickly after emitting only a few photons while others are far more robust and can undergo thousands or even millions of cycles before bleaching occurs.

Filters for Fluorescence Microscopy
The experimental setup in Figure 3.1 shows the typical filters used for epi-fluorescence microscopy, a form of microscopy in which both the excitation and emission light travel through the microscope objective. By carefully choosing the appropriate filters and mirrors for a given application, the signal-to-noise ratio can be maximized. As shown in Figure 3.1, three types of filters are used to maximize the fluorescence signal while minimizing the unwanted radiation. Each optical element is discussed below.

Excitation Filter
The excitation filter only allows a narrow band of wavelengths to pass through it, around the peak fluorophore excitation wavelength. For example, as shown in Figure 3.2, the bandpass region corresponding to greater than 90% transmission for the Yellow Fluorescent Protein (YFP) Excitation Filter (MF497-16) is 489 - 505 nm; incident radiation outside of this range is either partially (for regions near the transmission region) or totally (for regions further from the bandpass region) blocked by the filter.

Dichroic Mirror
Dichroic mirrors are designed to reflect light whose wavelength is below a specific value (i.e. the cutoff wavelength) while permitting all other wavelengths to pass through it unaltered. In a microscope, the dichroic mirror directs the proper wavelength range to the sample as well as to the image plane. The cutoff wavelength value associated with each mirror indicates the wavelength that corresponds to 50% transmission. For example, as shown in Figure 3.2, the cutoff wavelength for the Yellow Fluorescent Protein (YFP) Dichroic Mirror (MD515) is ~515 nm. The Specs tab provides information on the reflectance and transmission for each type of dichroic mirror.

By placing one of these mirrors into the experimental setup at 45° with respect to the incident radiation, the excitation radiation (shown in blue in Figure 3.1) is reflected off of the surface of the dichroic mirror and directed towards the sample and microscope objective, while the fluorescence emanating from the sample (shown in red in Figure 3.1) passes through the mirror to the detection system.

Although dichroic mirrors play a crucial role in fluorescence microscopy, they are not perfect when it comes to blocking unwanted light; typically, ~90% of the light at wavelengths below the cutoff wavelength value are reflected and ~90% of the light at wavelengths above this value are transmitted by the dichroic mirror. Hence, some of the excitation light can be transmitted through the dichroic mirror along with the longer wavelength fluorescence emitted by the sample. To prevent this unwanted light from reaching the detection system, an emission filter is used in addition to the dichroic mirror.

Emission Filter
An emission filter serves the purpose of allowing the desirable fluorescence from the sample to reach the detector while blocking unwanted traces of excitation light. Like the excitation filter, this filter only allows a narrow band of wavelengths to pass through it, around the peak fluorophore emission wavelength. For example, as shown in Figure 3.2, the bandpass region corresponding to greater than 90% transmission for the Yellow Fluorescent Protein (YFP) Emission Filter (MF535-22) is 524 - 546 nm; incident radiation outside of this range is either partially (for regions near the transmission region) or totally (for regions further from the bandpass region) blocked by the filter.

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Posted Comments:

Sashank Sriram (posted 2025-03-30 21:06:51.65)

Hi, I am curious if the dichroic mirrors presented here are polarisation sensitive. Since the mirrors are typically aligned at 45 degrees with the incoming beam of light, the electric fields can be either in-plane or out of the plane of the mirror. I would expect the two polarisations to interact differently with the components. Is there a significant difference in the performance of your dichroic mirrors versus the polarisation of light?

blarowe (posted 2025-03-31 03:37:27.0)

Thank you for contacting Thorlabs. Generally, we would expect some polarization dependence from these filters. I've reached out to you directly with more details.

Yuan Gong (posted 2023-12-28 12:08:46.28)

Hi, May I ask about the absorption of the filters, like MF525-39? I would like to buy a pair of this product, and I care about its reflection in the stop band. Can I roughly calculate the reflection at a specific wavelength by R=1-T? Many thanks! Have a nice one! Yuan

jpolaris (posted 2024-01-16 04:30:35.0)

Thank you for contacting Thorlabs. In general, R = 1-T is not a reliable estimate for reflectance in the stop band because it ignores the effects of absorption and interference which could be part of the blocking mechanism. I have reached out to directly with reflectance data.

Priyanka Jangra (posted 2023-09-13 22:19:46.323)

We want to know about the product FMF003 availability and price

jdelia (posted 2023-09-13 01:33:48.0)

Thank you for reaching out. FMF003 was obsoleted and replaced by MF535-22. You can find pricing and availability information for this product directly on the product family page.

Kevin Cappa (posted 2020-03-04 17:50:33.503)

If I had the option to get a 2"/50mm filter set for FITC fluorescent imaging I would...

nbayconich (posted 2020-03-05 01:03:35.0)

Thank you for your feedback, I will log your request in our internal engineering forum for future reference. We can provide custom sized filters upon request, I will reach out to you directly to discuss our custom capabilities.

tcohen (posted 2012-09-13 11:44:00.0)

Response from Tim at Thorlabs: Thank you for your interest in our custom capabilities. We should be able to provide this as a special and I have contacted you to go over your requirements.

densta (posted 2012-09-13 12:33:11.0)

Dear Madam/Sir I want to perform some preliminary fluorescence tests for a project of ours. I am interested in buying one of the following emission filters MF525-39: GFP MF530-43: FITC I noticed from your records that the outer diameter of the filter is 25 mm. In our system we need a filter with outer diameter 17 mm. The filter could be bare glass without an outer support ring. Is it possible to have one of the above mentioned filters in this form? What would be the cost? Best regards, Dimosthenis Stamopoulos

bdada (posted 2012-02-24 17:13:00.0)

Response from Buki at Thorlabs to Thank you for your interest in our imaging dichroic filters. Testing has shown no signs of degradation when exposed to at least 6 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours. Please contact TechSupport@thorlabs.com if you have any questions.

user (posted 2012-02-10 13:05:04.0)

Can you please provide laser damage threshold ratings for the dichroic filters?

jjurado (posted 2011-03-22 16:05:00.0)

Response from Javier at Thorlabs to flickingerd: Thank you for your feedback. Our filters follow that same convention as that of Semrock's. The arrow points to the recommended direction of light propagation. The reason for this is although the filter will function with either side facing the source, it is recommended to place the coated side toward the source. This will minimize any thermal effects or possible thermal damage that blocking intense out-of-band radiation might cause due to absorption by the substrate or colored glass filter layers.

lmorgus (posted 2011-03-21 18:23:00.0)

A response from Laurie at Thorlabs to flickingerd: As a follow-up to the response you received earlier today from Javier in our technical support group, I wanted to also reach out to you to thank you for your feedback. We have updated our web presentation to provide information about what the arrow on these optic mounts indicates. As a further step, we have made a list of other web pages on our site that need some attention for similar reasons. In the coming days, we will be updating those pages to clarify our engravings so that our customers can efficiently find the information they need to use our products effectively. Again, thank you for taking the time to drop us a note with a suggestion for improvement.

flickingerd (posted 2011-03-21 14:29:07.0)

I couldnt find info on proper filter orientation. There are arrows on the filters, but Ive seen different conventions on what these mean (Chroma says point arrow towards specimen for emission filters, yet Semrock convention is always point arrow in direction of light propagation, I think). How about your filters? This should be in "Tutorial" section, Id think. Please contact me with proper orientation rules. (Although Im still not sure exactly why this matters...I guess I can think of some minor reasons...)

apalmentieri (posted 2010-02-18 09:42:22.0)

A response from Adam at Thorlabs to pludowise: I believe we should be able to offer this item as a custom option. I would like to contact you directly to get more information about your application, the quantity necessary, and the exact size of the optic.

pludowise (posted 2010-02-18 00:01:38.0)

I am interested in finding a dichroic filter similar to MDF-TXRED, but shifted to loger wavelengths. Specifically, I need a dichroic for a Cy5 dye, which needs to transmit at 640 nm, and reflect at 690 nm. I also need this in a 1 mm thickness mounted at 45 deg AOI. Thank you.

12.5 mm Filter Sets

Zoom

Item #	Design Fluorophore	Excitation Band	Emission Band	Dichroic (Reflection / Transmission Band)
MDF05-BFP	Blue Fluorescent Protein (BFP)	395 ± 12.5 nm	460 ± 24.5 nm	350 - 418 nm / 433 - 750 nm
MDF05-GFP	Green Fluorescent Protein (GFP)	469.5 ± 20 nm	524.5 ± 24 nm	442 - 488 nm / 502 - 552 nm
MDF05-FITC	Fluorescein Isothiocyanate (FITC)	479 ± 15 nm	537 ± 20 nm	380 - 497 nm / 514.5 - 900 nm
MDF05-TOM	tdTomato	540 ± 12.5 nm	605 ± 27.5 nm	450 - 556.5 nm / 575 - 900 nm
MDF05-TXRED	Texas Red (TXRED)	562 ± 21 nm	635.5 ± 29 nm	460 - 587 nm / 608 - 900 nm
MDF05-MCHC	mCherry	560 ± 20 nm	629 ± 35.5 nm	450 - 578 nm / 593 - 700 nm
MDF05-CY5	Cy5	619 ± 30 nm	700 ± 35.5 nm	500 - 648 nm / 666 - 890 nm
MDF05-CY55	Cy5.5	650 ± 22 nm	720 ± 30 nm	520 - 674 nm / 692 - 1000 nm
MDF05-CY7	Cy7	709 ± 38 nm	813 ± 48 nm	570 - 746 nm / 766 - 1100 nm
MDF05-NIR	LI-COR IRDye 800CW	741 ± 20.5 nm	780 nm (Cut-On)	580 - 760 nm / 780 - 1100 nm

Click on for a plot and downloadable data.

The excitation and emission filter housings have an arrow engraved on them that points in the recommended direction of light propagation. Dichroic filters have a caret pointing to the side with the primary reflective coating. Light should be incident on this designated side of the optic for optimal performance.

Based on your currency / country selection, your order will ship from Newton, New Jersey

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MDF05-BFP NEW!BFP Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

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MDF05-GFP NEW!GFP Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

Today

MDF05-FITC NEW!FITC Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$401.25

Today

MDF05-TOM NEW!tdTomato Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$401.25

Today

MDF05-TXRED NEW!Texas Red Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$401.25

Today

MDF05-MCHC NEW!mCherry Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

Today

MDF05-CY5 NEW!Cy5 Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

Today

MDF05-CY55 NEW!Cy55 Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

Today

MDF05-CY7 NEW!Cy7 Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

Today

MDF05-NIR NEW!IRDye 800CW Ø12.5 mm Excitation, Ø12.5 mm Emission, and 12.5 mm x 17.6 mm Dichroic Filters

$518.95

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Ø25 mm Excitation Filters

Zoom

Item #	Design Fluorophore	Center Wavelength	FWHM
MF390-18	Blue Fluorescent Protein (BFP)	390 nm	18 nm
MF434-17	Cyan Fluorescent Protein (CFP)	434 nm	17 nm
MF445-45	Wild Type GFP (WGFP)	445 nm	45 nm
MF469-35	Green Fluorescent Protein (GFP)	469 nm	35 nm
MF475-35	Fluorescein isothiocyanate (FITC)	475 nm	35 nm
MF497-16	Yellow Fluorescent Protein (YFP)	497 nm	16 nm
MF542-20	Tetramethylrhodamine Isothiocyanate (TRITC)	542 nm	20 nm
MF559-34	Texas Red^® (TXRED)	559 nm	34 nm
MF565-24	Cy^®3.5 (CY3.5)	565 nm	24 nm

Click on for a plot and downloadable data.

These excitation fluorescence imaging filters are specifically designed to be used in microscopy and imaging applications. Each Ø25 mm filter is mounted in a 5 mm thick black anodized housing. The housing has an arrow engraved on it that points in the recommended light propagation direction.

These filters provide excellent transmission of the desired excitation wavelength (>90%), with a sharp spectral cutoff and low transmission at other wavelengths (<0.001%). Click on the More Info Icon icons above to view product-specific transmission data.

Based on your currency / country selection, your order will ship from Newton, New Jersey

+1 Qty Docs Part Number - Universal Price Available

MF390-18 BFP Ø25 mm Excitation Filter, CWL = 390 nm, BW = 18 nm

$337.05

Today

MF434-17 CFP Ø25 mm Excitation Filter, CWL = 434 nm, BW = 17 nm

$337.05

Today

MF445-45 WGFP Ø25 mm Excitation Filter, CWL = 445 nm, BW = 45 nm

$337.05

Today

MF469-35 GFP Ø25 mm Excitation Filter, CWL = 469 nm, BW = 35 nm

$337.05

Today

MF475-35 FITC Ø25 mm Excitation Filter, CWL = 475 nm, BW = 35 nm

$337.05

Today

MF497-16 YFP Ø25 mm Excitation Filter, CWL = 497 nm, BW = 16 nm

$337.05

Today

MF542-20 TRITC Ø25 mm Excitation Filter, CWL = 542 nm, BW = 20 nm

$337.05

Today

MF559-34 Texas Red Ø25 mm Excitation Filter, CWL = 559 nm, BW = 34 nm

$337.05

Today

MF565-24 Cy3.5 Ø25 mm Excitation Filter, CWL = 565 nm, BW = 24 nm

$337.05

Today

Ø25 mm Emission Filters

Zoom

Item #	Design Fluorophore	Center Wavelength	FWHM
MF460-60	Blue Fluorescent Protein (BFP)	460 nm	60 nm
MF479-40	Cyan Fluorescent Protein (CFP)	479 nm	40 nm
MF510-42	Wild Type GFP (WGFP)	510 nm	42 nm
MF525-39	Green Fluorescent Protein (GFP)	525 nm	39 nm
MF530-43	Fluorescein isothiocyanate (FITC)	530 nm	43 nm
MF535-22	Yellow Fluorescent Protein (YFP)	535 nm	22 nm
MF620-52	Tetramethylrhodamine Isothiocyanate/ Cy^®3.5 (TRITC/CY3.5)	620 nm	52 nm
MF630-69	Texas Red^® (TXRED)	630 nm	69 nm

Click on for a plot and downloadable data.

These emission fluorescence imaging filters are specifically designed to be used in microscopy and imaging applications. Each Ø25 mm filter is mounted in a 3.5 mm thick black anodized housing. The housing has an arrow engraved on it that points in the recommended light propagation direction.

These filters provide excellent transmission of the desired emission wavelength (>90%), with a sharp spectral cutoff and low transmission at other wavelengths (<0.001%). Click on the icons above to view product-specific transmission data.

25.0 mm x 36.0 mm Dichroic Filters

Zoom

Item #	Design Fluorophore	Reflection Band^b	Transmission Band^c
MD416	Blue Fluorescent Protein (BFP)	360 - 407 nm	425 - 575 nm
MD453	Cyan Fluorescent Protein (CFP)	423 - 445 nm	460 - 610 nm
MD480	Wild Type GFP (WGFP)	415 - 470 nm	490 - 720 nm
MD498	Green Fluorescent Protein (GFP)	452 - 490 nm	505 - 800 nm
MD499	Fluorescein isothiocyanate (FITC)	470 - 490 nm	508 - 675 nm
MD515	Yellow Fluorescent Protein (YFP)	490 - 510 nm	525 - 700 nm
MD568	Tetramethylrhodamine Isothiocyanate (TRITC)	525 - 556 nm	580 - 650 nm
MD588	Cy^®3.5/Texas Red^® (CY3.5/TXRED)	533 - 580 nm	595 - 800 nm

Click on for a plot and downloadable data.
Average reflection is >90% within this range.
Absolute transmission is >90% within this range.

Thorlabs' Dichroic Filters are designed to separate light of different wavelengths. When light is incident on the filter at a 45° angle with respect to the normal, the excitation light and its associated back reflection are reflected while the longer wavelength fluorescence signal is transmitted. These filters are unmounted, but are marked by a dash to indicate the coated side of the filter, which light should be incident on. Each filter is 25.0 mm x 36.0 mm. If your application would benefit from a round, mounted dichroic filter, consider our round Dichroic Filters. Click on the icons above to view product-specific transmission data.

Based on your currency / country selection, your order will ship from Newton, New Jersey

+1 Qty Docs Part Number - Universal Price Available

MD416 BFP 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 360 - 407 nm, Trans. Band = 425 - 575 nm

$326.35

Today

MD453 CFP 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 423 - 445 nm, Trans. Band = 460 - 610 nm

$326.35

Today

MD480 WGFP 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 415 - 470 nm, Trans. Band = 490 - 720 nm

$326.35

Today

MD498 GFP 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 452 - 490 nm, Trans. Band = 505 - 800 nm

$326.35

Today

MD499 FITC 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 470 - 490 nm, Trans. Band = 508 - 675 nm

$326.35

Today

MD515 YFP 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 490 - 510 nm, Trans. Band = 525 - 700 nm

$326.35

Today

MD568 TRITC 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 525 - 556 nm, Trans. Band = 580 - 650 nm

$326.35

Today

MD588 Cy3.5/Texas Red 25.0 mm x 36.0 mm Dichroic Filter, Refl. Band = 533 - 580 nm, Trans. Band = 595 - 800 nm

$326.35

Today

25 mm Filter Sets

Zoom

Item #	Design Fluorophore	Excitation Band	Emission Band	Dichroic (Reflection / Transmission Band)
MDF-BFP	Blue Fluorescent Protein (BFP)	390 ± 9 nm	460 ± 30 nm	360 - 407 nm / 425 - 475 nm
MDF-CFP	Cyan Fluorescent Protein (CFP)	434 ± 8.5 nm	479 ± 20 nm	423 - 445 nm / 460 - 610 nm
MDF-WGFP	Wild Type GFP (WGFP)	445 ± 22.5 nm	510 ± 21 nm	415 - 470 nm / 490 - 720 nm
MDF-GFP	Green Fluorescent Protein (GFP)	469 ± 17.5 nm	525 ± 19.5 nm	452 - 490 nm / 505 - 800 nm
MDF-FITC	Fluorescein Isothiocyanate (FITC)	475 ± 17.5 nm	530 ± 21.5 nm	470 - 490 nm / 508 - 675 nm
MDF-GFP2	Green Fluorescent Protein (GFP)/ Alexa Fluor^® 488	482 ± 9 nm	520 ± 14 nm	350 - 488 nm / 502 - 950 nm
MDF-YFP	Yellow Fluorescent Protein (YFP)	497 ± 8 nm	535 ± 11 nm	490 - 510 nm / 525 - 700 nm
MDF-TOM	tdTomato	531 ± 20 nm	593 ± 20 nm	350 - 555 nm / 569 - 950 nm
MDF-TRITC	Tetramethylrhodamine Isothiocyanate (TRITC)	542 ± 10 nm	620 ± 26 nm	525 - 556 nm / 580 - 650 nm
MDF-TXRED	Texas Red^® (TXRED)	559 ± 17 nm	630 ± 34.5 nm	533 - 580 nm / 595 - 800 nm
MDF-MCHC	mCherry	562 ± 20 nm	641 ± 37.5 nm	350 - 585 nm / 601 - 950 nm
MDF-CY3.5	Cy^®3.5	565 ± 12 nm	620 ± 26 nm	533 - 580 nm / 595 - 800 nm
MDF-MCHA	mCherry	578 ± 10.5 nm	641 ± 37.5 nm	350 - 588 nm / 603 - 950 nm
MDF-CY5	Cy5	619 ± 30 nm	700 ± 35.5 nm	500 - 648 nm / 666 - 890 nm
MDF-CY55	Cy5.5	650 ± 22 nm	720 ± 30 nm	520 - 674 nm / 692 - 1000 nm
MDF-CY7	Cy7	709 ± 38 nm	813 ± 48 nm	570 - 746 nm / 766 - 1100 nm
MDF-NIR	LI-COR IRDye^® 800CW	741 ± 20.5 nm	780 nm (Cut-On)	580 - 760 nm / 780 - 1100 nm

Click on for a plot and downloadable data.

Since standard fluorescence imaging applications generally incorporate three different filters (i.e., one excitation, one emission, and one dichroic filter) to maximize the signal-to-noise ratio, Thorlabs offers these filters as a set at a savings over purchasing each compenent separately. Some excitation, emission, and dichroic filters are only available as a matched set and are not available for purchase individually. These filters and filter sets are compatible with our 30 mm Cage Cubes for 25 mm filters. For customers who wish to use these filters in Thorlabs, Olympus, or Nikon fluorescence microscopes, Thorlabs manufactures a family of Drop-In Microscope Filter Cubes.

Please note some excitation and emission filter housings have an arrow engraved on them that points in the recommended direction of light propagation. Dichroic filters have an engraving on or a caret pointing to the side with the primary reflective coating. Light should be incident on this designated side of the optic for optimal performance.

Based on your currency / country selection, your order will ship from Newton, New Jersey

+1 Qty Docs Part Number - Universal Price Available

MDF-BFP BFP Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-CFP CFP Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-WGFP WGFP Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-GFP GFP Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-FITC FITC Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

In Stock Overseas

MDF-GFP2 GFP/Alexa Fluor 488 Ø25 mm Excitation, Ø25 mm Emission, and 25.2 mm x 35.6 mm Dichroic Filters

$1,143.36

Today

MDF-YFP YFP Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-TOM tdTomato Ø25 mm Excitation, Ø25 mm Emission, and 25.2 mm x 35.6 mm Dichroic Filters

$1,143.36

Today

MDF-TRITC TRITC Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-TXRED Texas Red Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-MCHC mCherry Ø25 mm Excitation (562 nm), Ø25 mm Emission, and 25.2 mm x 35.6 mm Dichroic Filters

$1,143.36

Today

MDF-CY3.5 Cy3.5 Ø25 mm Excitation, Ø25 mm Emission, and 25.0 mm x 36.0 mm Dichroic Filters

$920.20

Today

MDF-MCHA mCherry Ø25 mm Excitation (578 nm), Ø25 mm Emission, and 25.2 mm x 35.6 mm Dichroic Filters

$1,143.36

Today

MDF-CY5 Cy5 Ø25.0 mm Excitation, Ø25.0 mm Emission, and 25.0 mm x 35.8 mm Dichroic Filters

$989.75

Today

MDF-CY55 Cy5.5 Ø25.0 mm Excitation, Ø25.0 mm Emission, and 25.0 mm x 35.8 mm Dichroic Filters

$989.75

Today

MDF-CY7 Cy7 Ø25.0 mm Excitation, Ø25.0 mm Emission, and 25.0 mm x 35.8 mm Dichroic Filters

$989.75

Today

MDF-NIR IRDye 800CW Ø25.0 mm Excitation, Ø25.0 mm Emission, and 25.0 mm x 35.8 mm Dichroic Filters

$989.75

Today

32 mm Filter Sets

Zoom

Item #	Design Fluorophore	Excitation Band	Emission Band	Dichroic (Reflection / Transmission Band)
MDF32-BFP	Blue Fluorescent Protein (BFP)	395 ± 12.5 nm	460 ± 24.5 nm	350 - 418 nm / 433 - 750 nm
MDF32-GFP	Green Fluorescent Protein (GFP)	469.5 ± 20 nm	524.5 ± 24 nm	442 - 488 nm / 502 - 552 nm
MDF32-FITC	Fluorescein Isothiocyanate (FITC)	479 ± 15 nm	537 ± 20 nm	380 - 497 nm / 514.5 - 900 nm
MDF32-TOM	tdTomato	540 ± 12.5 nm	605 ± 27.5 nm	450 - 556.5 nm / 575 - 900 nm
MDF32-TXRED	Texas Red (TXRED)	562 ± 21 nm	635.5 ± 29 nm	460 - 587 nm / 608 - 900 nm
MDF32-MCHC	mCherry	560 ± 20 nm	629 ± 35.5 nm	450 - 578 nm / 593 - 700 nm
MDF32-CY5	Cy5	619 ± 30 nm	700 ± 35.5 nm	500 - 648 nm / 666 - 890 nm
MDF32-CY55	Cy5.5	650 ± 22 nm	720 ± 30 nm	520 - 674 nm / 692 - 1000 nm
MDF32-CY7	Cy7	709 ± 38 nm	813 ± 48 nm	570 - 746 nm / 766 - 1100 nm
MDF32-NIR	LI-COR IRDye 800CW	741 ± 20.5 nm	780 nm (Cut-On)	580 - 760 nm / 780 - 1100 nm

Click on for a plot and downloadable data.

Standard fluorescence imaging applications generally incorporate three different filters (i.e., one excitation, one emission, and one dichroic filter) to maximize the signal-to-noise ratio. These matched filter sets are designed to target common fluorophores and are compatible with our 30 mm Cage Cubes for 32 mm filters.

The excitation and emission filter housings have an arrow engraved on them that points in the recommended direction of light propagation. Dichroic filters have a caret pointing to the side with the primary reflective coating. Light should be incident on this designated side of the optic for optimal performance.

Based on your currency / country selection, your order will ship from Newton, New Jersey

+1 Qty Docs Part Number - Universal Price Available

MDF32-BFP NEW!BFP Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today

MDF32-GFP NEW!GFP Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today

MDF32-FITC NEW!FITC Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$925.55

Today

MDF32-TOM NEW!tdTomato Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$925.55

Today

MDF32-TXRED NEW!Texas Red Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$925.55

Today

MDF32-MCHC NEW!mCherry Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today

MDF32-CY5 NEW!Cy5 Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today

MDF32-CY55 NEW!Cy5.5 Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today

MDF32-CY7 NEW!Cy7 Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today

MDF32-NIR NEW!IRDye 800CW Ø32.0 mm Excitation, Ø32.0 mm Emission, and 32.0 mm x 44.0 mm Dichroic Filters

$1,310.75

Today