Thorlabs Fiber Photometry System Components

• Thorlabs' Fiber Photometry Equipment is Used Extensively in Leading Labs
• Custom Equipment Built to Order
• Complete System Including Fiber Optic Cannulae, Fiber Patch Cables, and Light Sources
• Stock Components (Same Day Shipping)
• Fiber Optic Cannulae
• Stereotaxic Cannula Holders and Adapter Arms
• Cannula Implant Guides
• Fiber Optic Patch Cables
• Fiber-Coupled LEDs
• Modulated LED Drivers
• High Bandwidth Detectors
• Low Autofluorescence Patch Cables
• Rotary Joints
• Lightweight Patch Cables
• Fiber Optic Interconnects and Mating Sleeves
• Fluorescence Filters and Dichroic Mirrors
• Multimode Fiber Optic Filter Cubes
• LED and Laser Light Sources
• Next-Day Availability for Custom Patch Cable Orders Placed Before 2 PM EST

Thorlabs offers a full line of equipment for in vivo stimulation, including implantable fiber optic cannulae, fiber optic patch cables, rotary joints, and LED and laser light sources. We are also well equipped to provide custom fiber photometry packages, including fiber-coupled light sources and custom-made cannulae. Please contact Tech Support for individual assistance regarding fiber photometry equipment selection.

The equipment needed for photometry is very similar to that used in an optogenetics experiment; however, due to the nature of fluorescence imaging, care must be taken in selecting the correct fiber optic components throughout the system to optimize signal intensity. In addition to the abovementioned common optogenetic tools, filters and dichroics with mounting hardware and multimode collimators used for fiber-to-free-space coupling are needed to filter and split the outgoing excitation signals from the incoming fluorescence signals. The collimators and rotary joints use an achromatic design to ensure that insertion loss is consistent throughout the visible wavelength spectrum that pertains to fiber photometry.

Interactive Fiber Photometry System Schematic

Click on the components for more details about our optogenetics line of products. Contact Tech Support for more information about our expanding line of fiber photometry products.

Introduction to Fiber Photometry

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An example of fluorescence data showing neural activity in a GCaMP transgenic sample. GCaMP fluoresces only when bound to a calcium ion, providing insight into calcium dynamics and neuronal response patterns.

Fiber photometry, a technique related to optogenetics, provides detailed insight into the activity and behavior of neuronal populations. This technique stimulates neurons with light and measures fluorescence signals that correspond to calcium dynamics. By detecting changes in the fluorescence intensity from a genetically encoded calcium indicator (GECI), calcium dynamics related to activities and patterns in neural circuits can be measured in real time.

The technique has helped in developing treatments for neurological diseases and brain-related traumatic injuries. Its use of light offers many advantages over other methods of neuromodulation, including a temporal resolution on the order of milliseconds, closely matching natural neuronal activity; and the stimulation of multiple neuron sets independently through the use of different wavelengths.

In fiber photometry, data is collected by analyzing the change in fluorescence (ΔF) relative to an initial baseline fluorescence (F) and observing a change in signal that corresponds to a calcium transient (ΔF/F). These indicators are typically based on fluorophores like GFP, RFP, tdTomato, mCherry, etc., of which GCaMP is the most common example. GCaMP contains Green Fluorescent Protein (GFP). As such, GCaMP not only exhibits the same fluorescence patterns as standalone GFP, but also provides key insight into calcium dynamics because GCaMP only fluoresces when bound to a calcium ion. In neurons, calcium ions regulate several important processes, including neurotransmitter release and membrane excitability. Therefore, GCaMP fluorescence is tied directly to neuronal response patterns, as depicted by the graph to the right.

To excite GCaMP in a neuronal population of interest, 470 nm and 405 nm LED sources are used to simultaneously photoexcite the maximum absorption and isosbestic points of the fluorophore, respectively. Each peak on a graph of ΔF/F indicates a neuronal response to an externally applied light stimulus. When using GCaMP as the fluorophore, as is most typical for fiber photometry, the peak specifically indicates when 525 nm fluorescence is emitted, following the simultaneous application of 405 nm and 470 nm inputs.

The main advantage of this excitation scheme is that excitation at the 405 nm isosbestic point will yield calcium-independent fluorescence in contrast to the calcium-dependent fluorescence at 470 nm. By modulating the excitation sources, the two emission signals can be read independently and simultaneously, providing important information about which part of the detected signal can be attributed to real information about behavior as opposed to background noise. This background noise can be generated from the fiber optic cables, interconnects, and cannulae used in the setup. Motion artifacts pose a particular risk because they create sharp features in recordings of ΔF/F that could easily be misunderstood as spikes in calcium activity if the 405 nm isosbestic point excitation is not measured in parallel.

Fiber Optic Cannulae

• Low-Profile Implantable Fiber Optic Cannulae
• Ø1.25 mm and Ø2.5 mm Ceramic and Stainless Steel Ferrules Available
• Ø200 µm, Ø300 µm, and Ø400 µm Multimode Optical Fiber Available
• Precision-Cleaved or Scissor-Cut Fiber End
• Custom Cannula Also Available (see the Custom Cannula Tab)

Thorlabs offers stock and custom fiber optic cannulae, which can be surgically mounted to the skull of the specimen using stereotactic guidance. Custom cannulae are available with stainless steel or ceramic ferrules as well as an array of different fiber types, lengths, and end terminations. See the Custom Cannula tab above for details.

For fiber photometry applications, we recommend using Ø400 µm, 0.50 NA optical fiber to maximize signal intensity and minimize autofluorescence intensity. As detailed below, we offer Low Autofluorescence Patch Cables that meet these specifications.

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Ferrule Patch Cables are ideal for connection to our Implantable Fiber Optic Cannulae.

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Fiber Optic Cannulae are available in various
fiber lengths and ferrule sizes.

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Implant Guide Assembly

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Weep Holes for Epoxy to Escape

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Mounting Surface of OGF Cannula Impant Guide

Cannula Implant Guides

• Improves Adhesion and Stability During Implantation
• Compatible with Ø1.25 mm and Ø2.5 mm Fiber Optic Cannulae with ≥5 mm Length
• Ø3.8 mm (OGL) or Ø5.1 mm (OGF) Mounting Surface with Grooved Ring for Dental Cement
• Lightweight Surgical Titanium Construction (≤0.11 g)
• Weep Holes for Glue and Epoxy to Secure a Cannula
• Compatible with Stereotaxic Cannula Holders

These Cannula Implant Guides are designed to provide guidance and stability for a fiber optic cannula during an implantation procedure. The bottom surface of each implant guide features a roughened surface and circular groove (see image to the right) that increase the surface area available to dental cement and improves adhesion to the specimen. A 1.6 mm long protrusion on the implant guide helps stabilize the guide when implanted. Each implant is constructed using lightweight surgical titanium (≤0.11 g) which can be sterilized prior to use.

For best results when implanting a cannula, the OGL and OGF should be used with a cannula holder and stereotaxic guidance equipment. To affix the cannula within the implant guide, first insert the cannula into the receptacle of the implant guide. Then, add a small amount of cement or epoxy to the cannula via the two Ø0.8 mm weep holes (see image above). Finally, attach the cannula ferrule to an XCL (Ø1.25 mm ferrule) or XCF (Ø2.5 mm ferrule) cannula holder (see image above).

The OGL implant guide is compatible with our standard Ø1.25 mm cannulae (ceramic and stainless steel) and the OGF implant guide is compatible with our standard Ø2.5 mm cannulae (ceramic and stainless steel). When assembled, the length of the protruding fiber is reduced by 1 mm (Item # OGL) or 2 mm (Item # OGF); therefore, these implant guides cannot be used with our 2 mm long cannulae. Additionally, due to the fiber separation distance, the implant guides cannot be used with our dual-core cannulae.

Fiber Optic Patch Cables

Multimode Step Index Patch Cables

• 0.50 Numerical Aperture
• Cables Available for Wavelengths from 250 nm to 2400 nm
• SMA905-to-SMA905 and SMA905-to-FC/PC Cables Available
• Ø3 mm Outer Jacket
• Custom Cables Available

Thorlabs offers multimode step index fiber optic patch cables with SMA905 (straight ferrule) and FC/PC connectors. These cables are ideal for a broad range of wavelengths from 250 nm to 2400 nm.

Each patch cable includes two protective caps that shield the connector ends from dust and other hazards. Additional CAPM Rubber Fiber Caps and CAPSM Metal Threaded Fiber Caps for SMA905-terminated ends are also sold separately. All patch cables on this page are sold from stock with same-day shipping available.

The majority of our 0.50 NA patch cables have orange (Ø3 mm) PVC furcation tubing, while the Ø1500 µm core fibers are packaged in stainless steel jackets. We recommend choosing stainless steel jackets when using fibers with large core diameters (≥Ø1000 µm) or high NAs (≥0.50) in light-sensitive applications, as it is easier for stray ambient light to penetrate the Ø3 mm (Item # FT030) fiber jackets. Alternatively, custom patch cables may be purchased that use our black or stainless steel furcation tubing (e.g., FT030-BK, FT038-BK, FT061PS, and others), in order to minimize stray light entering the fiber.

These cables are not designed for applications that require the fibers to carry high optical powers, as excessive powers can cause the epoxy used in the connectors to experience catastrophic heating. Please see the Damage Threshold tab for detailed information. Thorlabs offers alternate cabling options, in addition to unconnectorized fibers, that are compatible with high optical powers. Links to some options are included in the table below.

We have extensive patch cable capabilities including a large, diverse stock of optical fiber and many different types of fiber connectors. If you do not see a stock cable suitable for your application, please see our Custom Patch Cables webpage to request a cable that meets your specific needs.

Common Fiber-Coupled LEDs
Item #	M405FP1	M470F3a
Center Wavelength	405 nm	470 nm
Bandwidth (FWHM)	12 nm	20 nm
Typical Output Spectrum (Click for Graph)
Ø200 µm Core Fiber Output (Typ.)b	7.7 mW	7.0 mW
Ø400 µm Core Fiber Output (Typ.)c	24.3 mW	21.8 mW
CW Drive Current (Max)	1.0 A	1.0 A
LED Forward Voltage	3.45 V	3.1 V
Typical Lifetime	>40 000 Hours	>50 000 Hours

• This previous-generation item is no longer available for purchase.
• Tested using MM Fiber with Ø200 µm core, 0.22 NA (Item # FG200UCC).
• Tested using MM Fiber with Ø400 µm core, 0.39 NA (Item # FT400EMT).

Fiber-Coupled LEDs and Modulating LED Drivers

Neuron stimulation requires precise temporal control of the output light, typically on the order of millisecond pulses. For in vivo applications, electronic modulation of a fiber-coupled LED may be preferred over using a mechanical shutter or other modulation technique. Fiber-coupled LEDs at common wavelengths for fiber photometry can be seen in the table to the right. In a fiber photometry setup utilizing GCaMP as the fluorophore, a 470 nm LED, such as the previous-generation M470F3, and a 405 nm LED, such as the M405FP1, can be used.

Our fiber-coupled LEDs can be modulated in several ways when used with our LED drivers, such as the LEDD1B. The LEDD1B is designed to drive high-power LEDs with currents up to 1200 mA. It features an adjustable LED current limit to protect the connected LED. The output current can be limited continuously from 200 mA to 1200 mA using the adjuster on the front of the unit, thereby ensuring the output current does not exceed the limit regardless of the other settings or the modulation input voltage. A typical fiber photometry system will require two synchronized drivers that can simultaneously deliver light at both the isosbestic and maximum excitation wavelengths.

This LED Driver has the same compact form factor as other T-Cube modules, and, thus, it integrates well into the platform. It is shipped attached to a removable base plate, which allows the T-Cube to be easily secured to an optical table. The power supply options compatible with the LEDD1B LED Driver can be found here.

The DC4104 is another driver that is compatible with our fiber-coupled LEDs. This driver is capable of driving up to four of our fiber-coupled LEDs via the DC4100-HUB connector hub with a current range between 0 and 1000 mA. The current of each LED can be set individually by the driver or modulated individually by four external signals through the external modulation cable that comes included with the driver. Please contact Tech Support for additional cables. This driver can be operated through a wheel selector and three buttons on the front panel or remotely via USB 2.0 and the included software package.

Low-Autofluorescence Patch Cables

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Click Here for Raw Data
Plot comparing the recovery of autofluorescence for a low-autofluorescence (AF) and standard patch cable after photobleaching at 470 nm for 12 hours. A previous-generation M470F3 LED was used for excitation and autofluorescence intensity at 525 nm was measured relative to the output power from the patch cable.

Fluorescence signals are characteristically low, so it is important to maximize signal collection by selecting fibers and cannulae with large numerical apertures and core sizes. In general, Ø400 µm, 0.50 NA core fiber is recommended for adequate signal level, though, to minimize implant size in in vivo experiments, Ø200 µm core fiber can be used as well. Although smaller NAs such 0.39 NA can be used for some optogenetics applications, fibers with a 0.50 NA maximize signal intensity and are therefore recommended for fiber photometry. However, in any high NA fiber, the polymer cladding itself will exhibit some non-zero autofluorescence (AF). On the input side, filters should be used to ensure the input signal is clean, but on the output side, autofluorescence can be easily confused with fluorescence from the genetically encoded calcium indicator (GECI) being studied in the experiment since they are modulated at the same frequency.

To reduce these artifacts, Low-Autofluorescence Patch Cables should be used. These cables are manufactured with components and epoxies that reduce the emitted autofluorescence in the visible spectrum and should be photobleached before use to further minimize noise (see the graph below). This reduction makes these patch cables suitable for fiber photometry applications where high sensitivity is required to measure the changes in fluorescence that indicate neural activity within a specimen. As shown in the schematic above, low-autofluorescence cables are most important in the signal collection path of the fiber photometry system. The design of these patch cables is based on testing of the autofluorescent properties of our patch cable components, such as the bare fiber, ferrule types, and epoxies.

Each patch cable is 1 m long and incorporates a Ø400 µm, 0.50 NA multimode fiber (Item # FP400URT) and is available with three connector configurations. One end is equipped with an FC/PC connector, while the other is equipped with an FC/PC connector, Ø1.25 mm stainless steel ferrule, or a Ø2.5 mm stainless steel ferrule. Black jacketing along the cable minimizes light leakage. Each patch cable includes two protective caps that shield the ferrule ends from dust and other hazards when not in use. Additional plastic, metal, or threaded caps for the connector and ferrule ends are sold separately. If the fiber ends become dirty from use, we offer a selection of inspection tools, as well as fiber optic cleaning products. Similar to our standard optogenetics patch cables, the patch cables with a ferrule end can be mated to a fiber optic cannula using an interconnect or mating sleeve; see the selection guide for compatible products.

We can custom fabricate cables, including armored cable for protection from specimen damage and fan-out cables for incorporating multiple light sources into one fiber optic implant. If you do not see a stock cable suitable for your application, please see our Custom Patch Cables webpage to request a cable that meets your specific needs.

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The APD440A2 Avalanche Detector is recommended for fiber photometry applications.

High-Bandwidth Detectors

• Continuously Variable Gain
• Internal SM05 and External SM1 Threads Accept Most Fiber Adapters, Lens Tubes, and Other Components
• Power Supply Included

Thorlabs offers Avalanche Photodetectors (APD) for use in fiber optics systems. These devices feature a variable gain that can be controlled by a knob on the right side of the housing. SM1 threads are ideally matched with our internally SM1 threaded fiber adapters, such as S120-FC2 and S120-SMA, since they create a light-tight path that allows the fiber tip to be brought as close as possible to the detector element.

APDs provide the amplification and low noise-equivalent power (NEP) necessary to recover fiber photometry signals, which can be faint. With care taken, an index matching gel may be used to bring the fiber tip into optical contact with the window on the APD. For fiber photometry applications, we recommend the APD440A2. The 100 kHz bandwidth of this photodetector easily captures events at the millisecond time scales that are characteristic of transient fiber photometry signals. We also offer a range of high-pass electrical filters that can be matched to the repetition rate of your experiment.

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The RJ1 rotary joint has terminated ends and accepts a wide variety of FC/PC patch cables.

1x1 Rotary Joint for FC/PC Multimode Patch Cables

• Rotary Joint Protects Against Fiber Damage Caused by Moving Specimen
• Consistent Performance Over 400 - 700 nm Wavelength Range
• Low Transmission Variation During Rotation
• Recommended for ≥Ø200 µm Core, 0.22 to 0.50 NA Patch Cables
• Tested and Designed for Use with a Multimode Laser or Fiber-Coupled LED

Rotary Joint Commutator
For optogenetics applications and processes that utilize the same materials, such as fiber photometry, the intensity and stability of light that reaches a specimen is determined by how the transmission through a rotary joint changes with rotation. This can also be affected by the core diameter and NA of the connected patch cables, as mentioned previously, as well as by the wavelength and beam divergence of the light sources. When measuring fluorescence from a moving specimen, a fiber optic rotary joint can be used to ensure that the cables do not twist or get tangled, which would potentially damage the fiber optic components.

The collimators and RJ1 rotary joints are designed specifically for use with high NA fibers and use an achromatic design to ensure that insertion loss is consistent throughout the visible wavelength spectrum that pertains to fiber photometry. The selection of the filters and dichroics will depend upon the specific GECI being studied, with 470 nm (gCaMP) and 565 nm (rCaMP) being common examples.

The RJ1 rotary joint commutator has been shown not to add any fluorescence and has minimal rotational variation, which is critical when working with low signal levels. The achromatic design of the RJ1 ensures that insertion loss and rotational variation is very similar throughout the visible wavelength spectrum (400-700 nm), so again the isosbestic point can be used to differentiate motion artifacts from rotation from real calcium transients. The pigtailed rotary joints have higher rotational variation and are therefore not recommended for fiber photometry.

Interconnect and Mating Sleeves

• Ceramic Mating Sleeves for Ø1.25 mm or Ø2.5 mm Bare Ferrules (Sold Individually or in Packs of 5)
• Quick-Release Interconnect for Mating Ø1.25 mm or Ø2.5 mm Ferrule Patch Cables to Cannulae
• Compatible with Stainless Steel and Ceramic (Zirconia) Ferrules

Thorlabs offers interconnects and mating sleeves for making connections between our line of optogenetics patch cables and fiber optic cannulae. These ferrule mating components provide low-loss coupling and are compatible with both stainless steel and ceramic (zirconia) ferrules. Interconnects are designed to facilitate easy connections and disconnections from an implanted cannula, requiring >80% less force to disconnect compared to mating sleeves. On the other hand, mating sleeves are preferred for very lightweight (~0.18 g), low-profile connections between a patch cable and cannula.

Excitation and emission filters are marked with an arrow that shows the recommended direction of light propagation.

Dichroic filters are marked on the side with the dichroic coating. Light should be incident on this side for best performance.

Fluorescence Imaging Filters and Dichroics

• Excitation, Emission, and Dichroic Filters for Fluorescence Imaging
• >90% Transmission over Desired Wavelength Band
• Sharp Cutoff (T < 0.001%) Outside the Transmission Band
• Matched Filter Sets Designed to Target Many Common Fluorophores:
  

  - BFP - CFP - WGFP - GFP

  - FITC - Alexa Fluor® 488 - YFP - tdTomato

  - TRITC - Texas Red - mCherry - Cyanine (CY3.5)

• Excitation and Emission Filters are Mounted in Black-Anodized Housings
• Dichroic Filters are Unmounted
• Filter Sets are Available at a Savings Over Individual Filter Prices

These excitation, emission, and dichroic filters are designed specifically for use in fluorescence imaging applications. They are fabricated at industry-standard dimensions that make them compatible with filter cubes from all major manufacturers. We offer individual filters and filter sets targeted at common fluorophores: BFP, CFP, WGFP, GFP, FITC, Alexa Fluor^® 488, YFP, tdTomato, TRITC, Texas Red, mCherry, and Cyanine (CY3.5). In addition, the Fluorophores tab provides information on the alternative fluorophores suitable for these filters. These filters are also available pre-installed into our microscope filter cubes.

Filter Design
Our filters are manufactured to high-performance optical specifications and designed for durability. They are produced via multiple dielectric layers deposited on a high-precision, fused silica substrate. The substrate is ground and polished to ensure that the highest possible image quality is maintained. The resulting hard-coated optics consist of filter layers that are denser than those obtained from electron beam deposition techniques and that reduce water absorption while greatly enhancing the durability, stability, and performance of the filter. Each filter layer is monitored during growth to ensure minimal deviation from design specification thickness, ensuring overall high-quality filter performance.

Each filter is housed in a black anodized aluminum ring, which makes handling easier and enhances the blocking OD by limiting scattering. These filters can be mounted in our extensive line of filter mounts and wheels. As the mounts are not threaded, Ø1" retaining rings will be required to mount the filters in one of our internally-threaded SM1 lens tubes. For customers who wish to use these filters in Thorlabs, Olympus, or Nikon fluorescence microscopes, Thorlabs manufactures a family of Drop-In Microscope Filter Cubes.

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Two FOFD3(/M)-A Cubes can be combined with a C4W-CC Cage Cube Connector.

Multimode Fiber Optic Filter Cubes

• Kinematic Fiber Optic Filter Cube for Fiber Photometry, Fluorescence Measurements, or Multimode Wwavelength Division Multiplexing (WDM)
• Three Ports with Multimode Fiber Collimators
  • Achromatic: 400 - 700 nm 
  • Recommended for ≤0.5 NA Fibers, ≥Ø200 µm Core Diameter
  • FC/PC 2.2 mm Wide Key Connector
• One Free-Space Port
  • SM1 (1.035"-40) Internal Threads for Integration with a Power Meter or Ø1" Lens Tube
  • Four 4-40 Tapped Holes Enable 30 mm Cage System Compatibility
• Includes One DFM1T1 Insert for Holding Fluorescence Filter Sets
• Easily Swap Cube Inserts with Different Mounted Optics

The FOFD3(/M)-A Kinematic Multimode Fiber Optic Filter Cube combines high-throughput, pre-aligned fiber collimators with the flexibility to quickly and repeatably exchange filters and dichroics for a variety of applications that require combining or separating light by wavelength. Three ports of the FOFD3(/M)-A filter cube have fiber collimators for connecting an LED or laser via mulitmode fibers. The collimators provide achromatic performance from 400 to 700 nm and are recommended to be used with ≥Ø200 µm, ≤0.5 NA mulitmode mode fibers. The single free-space port has internal SM1 (1.035"-40) threads, compatible with Ø1" lens tubes or many of Thorlabs' Avalanche Photodetectors. Three plastic FC bulkhead dust caps and one snap-on SM1 plastic dust cap are included, ensuring a light tight environment for low signals.

Alternatively, for those systems that require more flexibility, Thorlabs also offers Kinematic Fluorescence Filter Cubes with four free-space ports. High-NA Achromatic Collimators can be integrated into SM1-threaded ports of the filter cubes using an AD15F adapter. This provides the flexibility to easily change filter sets, and the ability to further customize the system, while requiring the user to carefully align the fiber collimators for maximum signal. 

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DFM1L Kinematic Fluorescence Filter Cube

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F950FC-A Multimode Collimator

Light Sources

Thorlabs offers a variety of fiber-coupled light sources that can be used for in vivo stimulation. Our Fiber-Coupled LEDs offer a durable, economic solution with a variety of wavelength choices. Our Fiber-Coupled Lasers, Pigtailed Laser Diodes, and Benchtop Laser Diodes provide higher power at the cannula tip, and our Multichannel Light Sources offer a variety of wavelengths in a compact unit.

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Multimode Optical Fiber

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SMA905 Connector Components

Cable and Cannula Building Supplies

• Bare Optical Fiber, Ferrules, Tubing, Connectors, and Tools
• Build Custom Cannulae and Fiber Optic Patch Cables
• Connectorization Kit Provides all Necessary Tools
• Complete Connectorization Instructions Available for Download

Thorlabs stocks individual components for building custom fiber optic cannulae and patch cables, including optical fiber, ferrules, connectors, tubing, and connectorization tools. Our FN96A connectorization manual, which is free to download, gives clear instructions on how to add connectors to optical fiber.

Custom Fiber Optic Cannula

Thorlabs offers custom cannulae with the following options. Place a quote request through the following form to receive a quote for your custom cannula order.

Custom Cannula Worksheet:

Fiber Type: FG050LGA (Ø50 µm Core, 0.22 NA)FG050UGA (Ø50 µm Core, 0.22 NA)FG105LCA (Ø105 µm Core, 0.22 NA)FG105UCA (Ø105 µm Core, 0.22 NA)FG200LCC (Ø200 µm Core, 0.22 NA)FG200LEA (Ø200 µm Core, 0.22 NA)FG200UCC (Ø200 µm Core, 0.22 NA)FG200UEA (Ø200 µm Core, 0.22 NA)FP200URT (Ø200 µm Core, 0.5 NA)FT200EMT (Ø200 µm Core, 0.39 NA)FT300EMT (Ø300 µm Core, 0.39 NA)FG365LEC (Ø365 µm Core, 0.22 NA)FG365UEC (Ø365 µm Core, 0.22 NA)FT400EMT (Ø400 µm Core, 0.39 NA)FP400URT (Ø400 µm Core, 0.5 NA) Ferrule Size:  Ø2.5 mm (FC)  |  Ø1.25 mm (LC) (Custom length ferrules are also available. Contact Tech Support for details.) Ferrule Material:  Stainless Steel  |  Ceramic (Zirconia)  Fiber End:  Flat Cleave (tolerance of ± 0.5 mm)  |  Scissor Cut  Fiber Length (mm)*:  Length is specified as in the diagram above, with tolerance as noted. Quantity*: Comments:

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Keya
	Acceptable - Actual performance depends on individual experimental conditions.
	Compatible - Ideal, or nearly ideal, performance under most experimental conditions.
	Optimized - Optimal performance under all experimental conditions.

a. Absorption and emission spectra are unavailable if any is red.

The table below displays all of the fluorophores that are compatible with our filter sets. The filter set item numbers are listed across the top row and the fluorophores are listed down the first column. Scroll through the table to view fluorophore compatibility with our filter sets.

Click on the below to view the filter set transmission with the absorption and emission spectra of the fluorophore. The key to the right details the meaning of all check marks in the table below. Please note that absorption and emission spectra are unavailable if any is red.