Products Home荧光显微镜载玻片和对准靶
- 25.4 mm x 76.2 mm Fluorescent Slides, and Ø1" Fluorescent Disks
- Cage Alignment Plates for 16 mm, 30 mm, and 60 mm Cage Systems
- Available in Five Colors: Blue, Green, Yellow, Orange, and Red
- Made of Cast Acrylic Substrate
- Provides a Uniform Fluorescent Field Across the Surface Area
FSK5
Set of All Five Colored Slides
ADF1
Blue Alignment Disk with Target
ADF5
Red Alignment Disk with Target
ADF7
Green Alignment Disk with Hole in Disk Center
FSK3
Yellow Slide
CPA4
Orange 30 mm Cage Alignment Plate
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特性
- 评估并对准荧光成像系统
- 五种不同的荧光光谱与多种荧光团相似(请看荧光曲线图标签)
- 荧光载玻片尺寸25.4 mm x 76.2 mm，厚度1.7 mm
- 荧光对准盘Ø1英寸，厚度1.7 mm，刻有靶或孔，便于对准
- 橘色荧光笼式对准板，厚度1.7 mm，用于16 mm、30 mm和60 mm笼式系统
Thorlabs提供多种荧光显微镜载玻片和对准盘，用于宽场、共聚焦或多光子荧光成像系统。它们由浇铸亚克力基底制成，包含五种不同的颜色：蓝色、绿色、黄色、橘色和红色。它们的荧光光谱与多种荧光团的光谱类似；更多详情，请看荧光曲线图标签。这些载玻片和对准盘具有连续的荧光场，因此可以在使用包含荧光团的样品之前评估和调整视场、照明一致性和样品对准。
荧光显微镜载玻片兼容我们的样品支架，这些支架可以安装厚度为1.7 ± 0.5 mm的26 mm x 76 mm载玻片。每种载玻片可以单独出售，也可以以五个一包出售，或以载玻片套件出售，一个载玻片套件中包含五种颜色的载玻片。荧光对准盘可以通过Ø1英寸固定式光学元件安装座、透镜套管或30 mm笼板安装。对准盘可以单独出售，也可以以五个一包出售。荧光笼式对准板刻有同心圆且中间具有通孔，对准Thorlabs笼式系统的中心。这些嵌入式对准工具可单独购买，用于16 mm、30 mm和60 mm笼式系统。
可以定制尺寸和形状，最大尺寸为1英尺 x 2英尺，详情请联系技术支持techsupport-cn@thorlabs.com。
该数据是典型值；实际性能可能会因批次不同而有所差异。
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Raw Data
使用M365FP1 365 nm光纤耦合LED照射时，FSK1蓝色荧光载玻片的荧光光谱测量。ADF1蓝色荧光对准盘的荧光光谱与FSK1蓝色荧光载玻片的荧光光谱相同。
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Raw Data
使用旧款M470F3 470 nm光纤耦合LED照射时，FSK2绿色荧光载玻片的荧光光谱测量。ADF2绿色荧光对准盘的荧光光谱与FSK2绿色荧光载玻片的荧光光谱相同。
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Raw Data
使用M365FP1 365 nm光纤耦合LED照射时，FSK3黄色荧光载玻片的荧光光谱测量。ADF3黄色荧光对准盘的荧光光谱与FSK3黄色荧光载玻片的荧光光谱相同。
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Raw Data
使用M565F3 565 nm光纤耦合LED照射时，FSK4橘色荧光载玻片的荧光光谱测量。ADF4橘色荧光对准盘的荧光光谱与FSK4橘色荧光载玻片的荧光光谱相同。
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Raw Data
使用M565F3 565 nm光纤耦合LED照射时，FSK6红色荧光载玻片的荧光光谱测量。ADF5红色荧光对准盘的荧光光谱与FSK6红色荧光载玻片的荧光光谱相同。
该数据是典型值；实际性能可能会因批次不同而有所差异。
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Raw Data
FSK1(蓝色)、FSK2(绿色)、FSK3(黄色)、FSK4(橘色)和FSK6(红色)荧光载玻片在300 nm - 700 nm波段的透过率测量结果比较。ADF1(蓝色)、ADF2(绿色)、ADF3(黄色)、ADF4(橘色)和ADF5(红色)荧光对准盘的透过率与相应颜色的荧光载玻片的透过率相同。
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两块FSK1蓝色荧光载玻片的透过率结果比较。ADF1蓝色荧光对准盘的透过率与FSK1蓝色荧光载玻片的透过率相同。
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两块FSK2绿色荧光载玻片的透过率结果比较。ADF2绿色荧光对准盘的透过率与FSK2绿色荧光载玻片的透过率相同。
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两块FSK3黄色荧光载玻片的透过率结果比较。ADF3黄色荧光对准盘的透过率与FSK3黄色荧光载玻片的透过率相同。
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两块FSK4橘色荧光载玻片的透过率结果比较。ADF4橘色荧光对准盘的透过率与FSK4橘色荧光载玻片的透过率相同。
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两块FSK6红色荧光载玻片的透过率结果比较。ADF5红色荧光对准盘的透过率与FSK6红色荧光载玻片的透过率相同。
| Posted Comments: | |
Jagdish A Krishnaswamy
(posted 2024-02-28 14:37:44.29) Hello,
This is from Adiuvo Diagnostics Pvt Ltd, a Chennai-based medical device startup.
We are interested in the fluorescent microscope slides in Thorlabs's product line. However, could you please clarify if the fluorescence in these samples can be excited across a range of UV wavelengths. We are interested in testing specifically at wavelengths 365 nm, 395 nm, and 415 nm. cdolbashian
(posted 2024-03-13 04:34:13.0) Thank you for reaching out to us with this inquiry. We plot the fluorescence responsivity data which we have for these slides within the 'Fluorescence Graphs' tab above. For some of these wavelengths which you have provided, I see we have some test data, though not for all of them. I have contacted you directly regarding the compatibility of these slides with your experimental endeavors. Santhosh Mamidala
(posted 2023-09-11 10:40:46.357) I am looking for calibration slides for Nikon Ti2-E widefield microscope to measure fluorescence intensity. Does this FSK5 set can be used for this purpose to measure in DAPI/TRITC/FITC/Cy5? ksosnowski
(posted 2023-09-12 10:10:04.0) Hello Santhosh, thanks for reaching out to Thorlabs. Unfortunately, we do not batch-test these and we do not control for a specific tolerance of the fluorescence response of these slides. These are not intended for use as a spectral calibration reference but are rather for field of view, illumination consistency, and sample alignment assessment. The exact spectra varies between different physical units however which presents issues if used as a reference in this manner. Andrea Nardi
(posted 2023-08-30 08:04:13.33) Do you have any absorption spectrum of the slides? cdolbashian
(posted 2023-08-30 09:16:34.0) Thank you for reaching out to us with this inquiry! Unfortunately, all the data we have regarding these acrylic slides is presented on the various tabs above. We have not, at the time of posting, characterized full absorption spectra for each of these products. user
(posted 2023-08-04 11:25:06.127) Hello, I was wondering if you have spectra of the quantum efficiency of the slides? cdolbashian
(posted 2023-08-04 12:18:35.0) Thank you for reaching out to us with this inquiry. All of the data we have regarding these test targets are presented on the overview and graphs tabs of this web presentation. Yuri Sacha Correa Lopes da Silva
(posted 2023-06-07 14:40:46.55) Could you guys make a version of that product to 1550nm? It would be very useful to use two to align the cavities I use. cdolbashian
(posted 2023-06-21 11:07:17.0) Thank you for reaching out to us with this inquiry! We actually do make IR alignment tools, which can be found here:https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=3201
I have contacted you directly to discuss your particular application. Arjan Tibbe
(posted 2023-03-10 10:43:40.417) The fluorescence spectra listed on the website of the fluorescent microscope slides are not correct. Can you provide the real fluorescence spectra?
Thanks jgreschler
(posted 2023-03-16 04:38:25.0) Thank you for reaching out to Thorlabs. Some part-to-part variation is to be expected with these products, the graphs provided are examples of previously tested items in the product line. I've reached out to you directly to review the data you collected and discuss whether or not it falls within the normal operating bounds for this product. If it does not we can issue you a tested replacement. Alex Adams
(posted 2022-11-07 13:41:40.993) Dear support, What are the fluorescence lifetime's of each slides ? Thanks, Alex cdolbashian
(posted 2022-11-08 01:32:45.0) Thank you for reaching out to us Alex, unfortunately these particular targets are intended for qualitative measurements and alignment purposes. While we tolerance their dimensional properties tightly, we do not have lifetime data for this fluorescent acrylic. All performance data we have is on the product page and different "graph" tabs. Antonio Yu
(posted 2022-01-09 20:46:19.863) Dear support,
Could you please provide the life time of each slides ? Thanks.
BR,
Antonio jgreschler
(posted 2022-01-11 11:06:05.0) Thank you for reaching out to Thorlabs. Additional specifications can be requested by contacting Technical Support. I have reached out to you directly to discuss this topic further. Lothar Schmid
(posted 2021-06-30 09:50:51.8) Hi Thorlabs team,
I have a few questions regarding the fluorescent microscope slides, which I would like to use for intensity calibration of a fluorescent reader:
* How reproducible is the fluorescence intensity from batch to batch?
* Do the spectra change over time?
* Do the slides show bleaching of fluorophores?
* Is the fluorescence temperature-dependent, for a temperature range between 0 and 60 degC?
Thanks,
Lothar Schmid cdolbashian
(posted 2021-07-20 08:28:30.0) Thank you for your interest in Thorlabs products. These materials are, unfortunately, not characterized in this manner. All data we have for them is provided on the web page. I have reached out to your to discuss your application directly. Gary Riggott
(posted 2021-02-17 12:53:43.627) Hi-
The results I've gotten with my fluorimeter do not match your reference spectra very well and I'm wondering if you have any thoughts on this.
Blue Std:
My result shows a broad peak from about 400 to 480nm that doesn't exist in the spectrum for the standard.
Green Std:
I get a broad peak from 480 to about 560nm with a max at 501. The reference shows a well-defined big peak at 499nm.
Orange Std:
I get a broad peak from about 580 to 610 with a max at 587. The reference shows a minor peak at 612, and I don't have anything there.
Yellow Std:
My only peak is at 366nm. The reference shows a major peak at 493 and a minor peak at 517. This is the worst agreement between measured and expected among any of the standards.
Red Std:
I get a peak at 566 and a broad peak from about 580-630, with a max at 598. The reference shows peaks at 472, a big one at 586 and a broad peak from 620-625. I have no peaks near 472nm.
I welcome any input.
If you could add a second "Run" or spectrum as you did with the %Transmission curves, that would be a huge help!
Thank you,
Gary YLohia
(posted 2021-04-14 02:36:20.0) Hello Gary, thank you for contacting Thorlabs. After going over the data you sent us and talking to our team here, it seems like the discrepancy that you are seeing ranges from 3 - 10 nm, which can be attributed to a lot-to-lot variation. These are not intended for use as a calibration spectra but are rather for field of view, illumination consistency, and sample alignment assessment. Vincent Khau
(posted 2020-12-01 18:18:29.887) Do you have data on the fluorescence variation from batch to batch for the blue fluorescence slide? Is there a certain tolerance that these slides are manfuactured to in terms of their fluorescence response?
Thank you. YLohia
(posted 2020-12-15 02:49:19.0) Hello, thank you for contacting Thorlabs. Unfortunately, we do not batch-test these and we do not control for a specific tolerance of the fluorescence response of these slides. Dmitry Gorbachev
(posted 2020-02-21 03:38:27.007) Hello! I work with Frequency-Domain FLIM, and I used your fluorescent slides kit FSK5 to tune our FLIM camera. I want to know if it is possible to order the same set of slides from you, but larger? for example 10cm in diameter. Thanks a lot! llamb
(posted 2020-02-25 02:12:46.0) Thank you for contacting Thorlabs. Yes, custom fluorescence slides are indeed feasible. We will reach out to you directly to quote this. Nikola Krstajic
(posted 2019-07-12 09:39:00.04) I work in near-infrared and fluorescence targets geared for ICG exc/emission (785nm exc) would really help me and all colleagues working in this are. I am 100% sure you would sell many. It is also not clear if your fluorescence targets work on 625nm excitation as your spectra show wavelengths below exc and this is really unusual (antistokes is an exception in fluorescence). Thanks a lot! YLohia
(posted 2019-07-23 09:46:29.0) Hello, thank you for your feedback. I have posted your request on our internal engineering forum for further consideration as a future product. petur.hermannsson
(posted 2019-01-21 10:33:44.437) Do you have numbers on the batch-to-batch consistency/uniformity of these slides, as well as the rate of photobleaching? YLohia
(posted 2019-01-28 11:03:57.0) Hello, thank you for contacting Thorlabs. We have actually just updated our web presentation to include some information on the consistency between two runs, and this information can be found in the "Transmission Graphs" tab. That being said, unfortunately, we do not have information on the photobleaching at the moment. |
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FSK5载玻片套件放在储存盒中以五个一包出售。
- 提供五种颜色：蓝色、绿色、黄色、橘色和红色
- 每种载玻片单独出售，或以1包5个出售
- FSK5载玻片套件包含五种颜色的载玻片
- 25.4 mm x 76.2 mm，厚度1.7 mm
- 兼容样品支架，用于安装厚度为1.7 ± 0.5 mm的26 mm x 76 mm载玻片
- FSK5载玻片套件和五块装的载玻片具有带刻记的储存盒
我们的荧光显微镜载玻片用于在荧光成像系统中评估和对准样品位置与照明水平。每块载玻片的尺寸为25.4 mm x 76.2 mm，厚度为1.7 ± 0.5 mm，由浇铸亚克力材料制成。FSK5载玻片套件和五块装的载玻片都附带塑料储存盒，以便保持清洁无尘。盒子上方刻有Thorlabs logo和产品型号，如右图所示。如需定制最大尺寸在1英尺 x 2英尺内的款式，请联系技术支持techsupport-cn@thorlabs.com。
这些载玻片兼容我们的样品支架，能够安装厚度≥1.7 mm的26 mm x 76 mm载玻片。请注意，我们的C4SH01四位置显微镜载玻片安装架不兼容这些载玻片。
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ADF5红色荧光对准盘安装在LMR1安装座中
- 提供五种颜色的荧光对准盘：蓝色、绿色、黄色、橘色和红色
- 每种对准盘单独出售，或以1包5个出售
- Ø1英寸，厚度1.7 mm
- 刻有靶参考线、Ø3.4 mm和Ø9.4 mm同心圆
- 兼容Ø1英寸固定式光学元件安装座、透镜套管或30 mm笼板
这些带靶参考线的对准盘用于辅助对准荧光成像系统。每个对准盘的直径为1英寸，厚度为1.7 ± 0.5 mm。盘上刻有靶和两个同心圆，以便对准。如需定制最大尺寸在1英尺 x 2英尺内的对准盘，请联系技术支持techsupport-cn@thorlabs.com。
这些对准盘可以安装在Ø1英寸固定式光学元件安装座、透镜套管或30 mm笼板中，如右图所示。
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ADF9橘色荧光盘安装在CP33T笼板中
- 提供五种颜色的荧光对准盘：蓝色、绿色、黄色、橘色和红色
- 每种对准盘单独出售，或以1包5个出售
- Ø1英寸，厚度1.7 mm
- 对准盘Ø1.5 ± 0.15 mm的中心孔用于对准
- 兼容Ø1英寸固定式光学元件安装座、透镜套管或30 mm笼板
我们的带孔荧光对准盘用于对准荧光成像系统。每个对准盘的直径为1英寸，由浇铸亚克力材料切割而成，厚度为1.7 ± 0.5mm。每个对准盘中心有一个Ø1.5 mm的孔。如需定制最大尺寸在1英尺 x 2英尺内的对准盘，请联系技术支持techsupport-cn@thorlabs.com。
这些对准盘可以安装在Ø1英寸固定式光学元件安装座、透镜套管或30 mm笼板，如右图所示。
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这些嵌入式对准板是用于空间校准和基于笼式荧光成像系统对准的简便工具。浇铸亚克力材料具有类似橘色荧光蛋白和染料的荧光光谱。这些对准盘具有连续的荧光场，因此可以在使用含有荧光团的样品之前评估和调整视场、照明一致性和样品对准。
每个对准盘由厚度为1.7 mm的浇铸亚克力材料切割而成，且带有一个Ø1 mm或Ø5 mm的通孔，对准笼式组件中心。每个通孔周围刻有同心圆对准环。提供与我们的16 mm、30 mm和60 mm笼式系统兼容的选项。如需定制颜色和1英尺 x 2英尺的最大尺寸，请联系技术支持techsupport-cn@thorlabs.com。
| Item # | Color | Through Hole Diameter | Alignment Ring Diameters | Compatible Cage System | Thickness | Transmission (Click for Graph) | Requires Charging |
|---|---|---|---|---|---|---|---|
| SCPA2 | Orange | 1 mm (0.04") | 3 mm, 5 mm, 7 mm, and 9 mm | 16 mm | 1.7 mm ± 0.5 mm |  | No |
| CPA3 | 1 mm (0.04") | 4 mm, 7 mm, 10 mm, and 13 mm | 30 mm | ||||
| CPA4 | 5 mm (0.20") | 7 mm, 10 mm, and 13 mm | 30 mm | ||||
| LCPA2 | 1 mm (0.04") | 7 mm, 12 mm, 17 mm, 22 mm, and 27 mm | 60 mm |
Additional Microscopy Targets
 荧光载玻片和对准盘 |