Common Fluorphores that can be Excited by the Octavius-2P
- Fluo-3 and Fluo-5F
- e and w Type GFP
- Alexa Dyes
- Oregon Green
- Cy2 and Cy3
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- Ultra-High Peak Power (>500 kW) for Deep Imaging
- >100 nm Wide Spectrum Allows Multiple Fluophores to be Excited Simultaneously
- 10 Femtosecond Pulse Provides More Signal and Less Photodamage
- Small Footprint Conserves Lab Bench Space
More Signal, Less Photodamage
In two-photon microscopy, the fluorescence signal intensity depends quadratically on both the excitation laser intensity and average power divided by repetition rate and laser pulse width:
Here, Pave is the average power of the femtosecond laser, frep is the repetition rate, and is the pulse length.
The two-photon signal can be increased either by increasing the average power of the femtosecond laser or by reducing the pulse width. Increasing the average power requires a high-power pump laser, which is expensive to buy and exhibits a high cost of ownership.
The Octavius-2P offers a cost-effective alternative. Instead of incorporating a multi-watt average output power system, we reduced the pulse width to 10 fs, thereby increasing the intensity tenfold compared to 100 fs systems with the same average power. The result is better signal intensity with reduced average laser power, which decreases the probability of photodamage.
The Octavius-2P is pumped using newly developed Optically Pumped Semiconductor Laser (OPSL) technology. These next-generation pump sources allow for high compactness and low cost of ownership. The ultra-high peak power of over 500 kW, compared to other commercially available 300 kW lasers, provides the ability to probe deeper into biological tissue. Additionally, the extended spectral bandwidth of the 10 fs laser pulse stretches over 100 nm, allowing the Octavius-2P laser to efficiently excite multiple fluorophores simultaneously.
|The Octavius-2P is under developent and will be available soon. For more information, please contact ImagingSales@thorlabs.com|