Create an Account  |   Log In

View All »Matching Part Numbers


Your Shopping Cart is Empty
           

Telesto 1325 nm OCT System


Telesto 1325 nm OCT System

TELESTO System Specifications
Center Wavelength1325 nm
Imaging Speed
(A-Scan Line Rate)
High-Speed Mode: 91 kHz
Video-Rate Mode: 28 kHz
High-Sensitivity Mode: 5.5 kHz
Axial (Depth) Resolution (Air)<7.5 µm
Lateral Resolution15 µm
Maximum Field of View10 mm x 10 mm x 2.5 mm
Maximum Pixels Per A-Scan512
OCT Systems Comparison
Download a Brochure

Application ArticlesApplication Articles

Thorlabs' Variable-Rate TELESTO™ OCT Imaging System

Features

  • 1325 nm Center Wavelength Provides Deep Image Penetration in Highly Scattering Samples
  • Three Acquisition Modes for Flexibility in Imaging Speed and Sensitivity
  • Ideal for High-Speed and Biological/Biomedical Imaging Applications
  • High Axial Resolution: Less than 7.5 µm

Thorlabs' TELESTO™ OCT Imaging System is the newest member of our SD-OCT imaging family. This system provides the flexibility required for high-speed, deep penetration, and high resolution imaging applications. It utilizes Thorlabs' unique superluminescent diode design, which provides over 150 nm of bandwidth, resulting in an axial resolution of less than 7.5 µm in air. In the software, users can select between three imaging modes to image up to 91,000 A-Scans per second with 91 dB sensitivity, down to 5,500 A-Scans per second with 106 dB sensitivity. Increasing the sensitivity, at the cost of imaging speed, enables detection of more subtle structures in a sample. High-speed imaging at 91 kHz enables rapid volume acquisition and display. Click here to see images of a fingertip taken at all three speed settings. Image size: 4.8 mm x 2.6 mm.

The TELESTO is controlled via software preinstalled on a high-performance computer that is capable of displaying greater than 6 volumes per second. The system also includes a 3D scanning, common path probe with integrated video camera for volume imaging and live video display. The included stand and sample stage provides XY translation and rotation of the sample along with axial travel of the probe. The TELESTO is fully functional right out of the box.

Optical Coherence Tomography (OCT) is a noninvasive optical imaging modality that provides real-time, 1D depth, 2D cross-sectional, and 3D volumetric images with micron-level resolution and millimeters of imaging depth. OCT images provide structural information of a sample, based on light backscattered from different layers of material within that sample. OCT imaging is considered to be the optical analog to ultrasound. OCT, however, achieves higher resolution through the use of near infrared wavelengths, at the cost of decreased penetration depth.  In addition to high resolution, the non-contact, noninvasive advantage of OCT makes it well suited for imaging samples such as biological tissue, small animals, and industrial materials.

Click here for a full list of peer-reviewed publications using Thorlabs OCT imaging products.

OEM and Custom Requests
Thorlabs offers OEM products for easy integration into your imaging system. With ISO9001 certified production facilities and experienced OEM design engineers, we welcome OEM requests.

We also recognize that our customers have unique application requirements. For this reason, we stand ready to discuss how our OCT systems can be adapted to meet your needs. We encourage you to send us samples for product testing so our engineers can customize one of our OCT systems to suit your individual applications. Please use the "Contact Us" button on the upper right-hand side of the page for more information.

Click on the Telesto OCT Imaging System Components, shown below, for further details.

Complete TELESTO System

 

 

 

TELESTO Processor

The TELESTO OCT System includes a desktop PC and a 22" monitor which has 1680 x 1050 resolution. This processing system is set up with all the necessary data acquisition hardware, drive electronics, and software to begin imaging upon arrival.

Computer Specifications*

  • Processor: Quad Core
  • Processor Speed: ≥ 3.3 GHz
  • Memory: ≥ 8 GB
  • Hard Drive: ≥ 500 GB
  • Data Acquisition: CameraLink

*Computer Type Subject to Change without Notice

Click Here to return to the top of the page.

 

TELESTO SD-OCT Engine

The TELESTO SD-OCT engine is housed in a 420 mm x 320 mm x 149 mm (16.54" x 12.6" x 5.86") unit. It contains Thorlabs' Matched Pair Broadband 1325 nm Superluminescent Diode Light Source and Linear InGaAs Array-Based Spectrometer. It also includes all associated drive electronics and controllers.

Click Here to return to the top of the page.

 

High Performance Software

High-performance ThorImage OCT data acquisition software is included with all SD OCT systems. The Windows-based software performs data acquisition, processing, scan control, and display of OCT images. See the Software tab for more details. A Software Development Kit (SDK) is also available for C/C++, Visual Basic, and LabVIEW-based interfaces. Please contact oct@thorlabs.com for more details.

Click Here to return to the top of the page.

 

Hand-Held Probe and Stand

All Thorlabs OCT systems include a hand-held probe and stand, as shown here. The probe provides X-Y scanning for three-dimensional data acquisition. A camera that is integrated in the probe provides live video imaging during OCT data acquisition. The probe easily slips onto the stand for imaging of small samples.
The probe stand consists of a post-mounted focus block which is attached to a specially designed 12" x 14" aluminum breadboard using a Ø1.5" P14 Post.

Stand Features

  • Ideal for Vibration-Sensitive Studies such as Doppler OCT Imaging
  • 3/4" Thick Aluminum Breadboard Provides Increased Stability
  • Breadboard Base has Side Grips and Recessed Feet for Easy Lifting and Transportation of the Stand
  • Includes a Sample Stage with 1" X and Y Travel as well as Rotation

Click Here to return to the top of the page.

ComputerOCT Engine and Imaging ModuleUser SoftwareHand-Held OCT Probe and Stand 

Thorlabs offers a wide variety of OCT imaging systems, each with their own special set of specifications. To assist in narrowing down which OCT system(s) is best suited for your application, we have provided the guide below. This information is intended only as a guide. We encourage you to contact us to discuss your specific imaging requirements.

Contact Us

 CALLISTOGANYMEDE-IIGANYMEDE-II-HRTELESTO-IITELESTO-II-1325LROCS1310V1
Center Wavelength930 nm (840 nm option also available)900 nm1300 nm1325 nm1300 nm
Bandwidth100 nma100 nma150 nma170 nma100 nma100 nmb
A-Scan Line Rate1.2 kHz36 kHzUp to 76 kHz100 kHz
B-Scan Frame Ratec,d
2 fps29 fpsVariable110 fps
Depth Resolutione (Air)7 µm
6 µm
3 µm
5.5 µm12 µm16 µm
Depth Resolutione (Water)5.3 µm4.3 µm2.1 µm4.2 µm9.0 µm12 µm
Lateral Resolution8 µm8 µm4 µm13 µm 25 µm
Sensitivity105 dB91 dBUp to 103 dB105 dB
Maximum Pixels per A-Scan512102410242048
Max Field of Viewe (L x W x D)10 x 10 x 1.7 mm10 x 10 x 2.9 mm6 x 6 x 1.9 mm10 x 10 x 3.5 mm10 x 10 x 7 mm10 x 10 x 12 mm
  • Bandwidth at 3 dB
  • Bandwidth at 20 dB
  • Actual Frame Rate is dependent on processing and display parameters
  • (512 lines/frame)
  • Depth Resolution and actual imaging depth are dependent on the optical properties of the sample being imaged.

ThorImage OCT Software Index

 

OCT Scan Control Screen Shot
Click to Enlarge

The ThorImage OCT window in the Volume Rendering Mode.

ThorImage OCT Software

  • Interactive Scan Position Control through Video Display (Draw and Scan)
  • Advanced Dataset Management
  • High-Speed Volume Rendering of 3D Data
  • Doppler and Speckle Variance Imaging
  • Versatile Scan and Acquisition Control

ThorImage OCT is high-performance data acquisition software that is included with all Thorlabs Spectral Domain OCT systems. This 64-bit Windows-based software package is capable of data acquisition, processing, scan control, and displaying OCT images. Additionally, NI LabVIEW and C-based Software Development Kits (SDKs) are available, which contain a complete set of libraries for measurement control, data acquisition and processing, as well as storage and display of OCT images. The SDKs provide the means for developing highly specialized OCT imaging software for every individual application.

OCT Scan Control Screen Shot
Click to Enlarge

Various acquisition parameters can be adjusted in ThorImage OCT.

Scan Control

ThorImage OCT provides numerous scan and acquisition controls. The camera integrated in the probe of our OCT system provides live video images in the application software. Defining the scan line for 2D imaging or the scan area for 3D imaging is accomplished through the easy-to-use "Draw and Scan" feature by clicking on the video image. The scan pattern can also be adjusted by specifying parameters in the controls of the software. Additionally, one can further set processing parameters, averaging parameters, and the speed and sensitivity of the device using device presets. By using a high-speed preset, video-like frame rates in 2D and fast volume rendering in 3D are possible, whereas high-sensitivity acquisition is enabled by choosing a preset with a lower acquisition speed.

High Speed OCT Software Draw and Scan
Click to Enlarge

The Sample Monitor can be used to define the scan pattern using the "Draw and Scan" feature.

ThorImage OCT Dataset Management Panel
Click to Enlarge

The Dataset Management Window of ThorImage OCT.

Dataset Management

ThorImage OCT provides advanced dataset management capabilities, which allow opening several datasets simultaneously. Datasets are uniquely defined using an identifier consisting of study (or test series) name and an experiment number. Grouping of datasets is possible using the same study name. The "Captured Datasets" list shows an overview of all open datasets, including the study name, the acquisition mode, and preview pictures of the still video image and the OCT data.

Datasets can be exported in various image formats, such as PNG, BMP, JPEG, PDF, or TIFF. Formats suited for post-processing purposes, such as RAW/SRM, FITS, VTK, VFF, and 32-bit floating-point TIFF, are also supported.

The OCT file format native to ThorImage OCT allows OCT data, sample monitor data, and all relevant metadata to be stored in a single file. ThorImage OCT can also be installed and run on computers without an OCT system installed in order to view data.

Third Party Applications

If ImageJ is installed on the computer with ThorImage OCT installed, opening acquired OCT data in ImageJ is one mouse click away, as shown in the image below. This enables a flawless workflow when requiring the advanced image processing functionality provided by ImageJ. Clicking the Explorer button shown below will open the folder and select the file in Windows Explorer where the currently active dataset is stored.

ThorImage OCT Third Party Applications Panel
Export buttons are accessible in the Action Toolbar of ThorImage OCT.

 

OCT Software 2D Mode
Click to Enlarge

The ThorImage OCT window in the 2D Mode.

2D Mode

In the 2D imaging mode the probe beam scans in one direction, thus acquiring cross-sectional OCT images, which are then displayed in real time. Line averaging before or after the Fast Fourier Transform (FFT) is available as well as B-Scan averaging. Image display parameters, such as color mapping, can be controlled in this mode. We have also implemented an option for automatic calculation of the optimum contrast and brightness of the displayed OCT images.

 

3D Mode

In the 3D imaging mode, the OCT probe beam scans sequentially across the sample to collect a series of 2D cross-sectional images, which are then processed to build a 3D image.

In the ThorImage OCT software, 3D volume datasets can be viewed as orthogonal cross-sectional planes (see below) and volume renderings.

The Sectional View features cross-sectional images in all three orthogonal planes, independent of the orientation in which the data was acquired. The view can be rotated as well as zoomed in and out.

The Rendering View provides a volumetric rendering of the acquired volume dataset. This view enables quick 3D visualization of the sample being imaged. Planes of any orientation can be clipped to expose structures within the volume. The 3D image can be zoomed in and out as well as rotated. Furthermore, the coloring and dynamic range settings can be adjusted.

Utilizing the full potential of our high-performance software in combination with our high-speed OCT systems, we have included a Fast Volume Rendering mode in ThorImage OCT, which serves as a preview for high-resolution 3D acquisitions. In this mode, high-speed volume renderings can be displayed in real-time, providing rapid visualization of samples in three dimensions.

OCT 3D Imaging Screen Shot
Click to Enlarge

Rendering View
OCT 3D Imaging Screen Shot
Click to Enlarge

Sectional View

 

OCT Doppler Imaging Screen Shot
Click to Enlarge

Doppler dataset showing the velocity of a rotated plastic stick.

Doppler Mode

Doppler OCT imaging comes standard with all OCT systems. In the Doppler mode, phase shifts between adjacent A-scans are averaged to calculate the Doppler frequency shift induced by particle motion or flow. The number of lateral axial pixels can be modified to change velocity sensitivity and resolution during phase shift calculation. The Doppler images are displayed in the main window with a color map indicating forward- or backward-directed flow, relative to the OCT beam.

 

OCT Doppler Imaging Screen Shot
Click to Enlarge

Speckle variance measurement showing blood vessels of a hand.

Speckle Variance Mode

ThorImage OCT 4.0 introduces a new acquisition mode, which uses the variance of speckle noise to calculate angiographic images. It can be used to visualize three dimensional vessel trees without requiring significant blood flow and without requiring a specific acquisition speed window. The speckle variance data can be overlaid on top of intensity pictures providing morphological information. Different color maps can be used to display the multimodal pictures.

 

Video Showing Screencast of ThorImage OCT Rendering Capabilities

In this video, OCT images of a finger are acquired and manipulated in the 3D volume and cross section modes.

Doppler OCT Imaging

Doppler OCT is an extension of OCT that enables imaging of particle motion within a sample. In Fourier Domain OCT (FD-OCT) systems, there are no additional hardware requirements for implementation of Doppler imaging. Doppler OCT imaging capability is embedded in the software provided with all Thorlabs’ OCT systems and is ideal for functional vascular imaging, studying embryonic cardiac dynamics, or monitoring vascular treatment response. It is also useful for general flow velocimetry used in microfluidic channel monitoring.

Principles of Doppler OCT

The Fourier transform of the interferogram acquired in FD-OCT imaging (A-scan) produces a complex signal [I(z) + iQ(z)], where the magnitude of that signal is used to create the structural OCT image. The complex portion of the signal contains information based on the phase of the interferogram. Any change in phase between consecutively acquired A-scans can be attributed to a Doppler frequency shift induced by particle motion.

In Thorlabs’ implementation of Doppler OCT, Doppler frequency shifts are calculated based on spatially averaging phase shifts within a sliding 2D window and using a Kasai autocorrelation function (see reference below). The Doppler frequency shift fD caused by moving particles is related to the phase shift between A-scans, as described in the following expression:

Equation 1

where Δφ is the average phase shift within the sliding 2D window and fA is the A-scan rate of the OCT system. The Thorlabs Doppler Imaging Mode displays the phase shift induced by moving particles using a standard Doppler colormap where red to yellow (violet to blue) indicates flow in the direction toward (away from) the OCT sample beam.

The mean velocity <ν> of the moving particles, at any depth, can be quantified by knowing θ, the angle between the OCT sample beam and flow vector:

Equation 2

Here, λo is the center optical wavelength of the OCT sample beam and n is the index of refraction of the sample.

Reference:
C. Kasai, K. Namekawa, A. Koyano, R. Omoto
et al . “Realtime two-dimensional blood flow imaging using an autocorrelation technique,” IEEE Trans. Sonics. Ultrason. 32 458-464 (1985).

Doppler Effect

A stationary observer will observe a Doppler shift in the frequency of light emitted from a source moving toward or away from the observer.When the light source is moving toward the observer, the observed frequency of light will be blue-shifted, meaning the perceived frequency of the light will be higher than the actual frequency emitted by the source. Higher frequencies correspond to shorter wavelengths. Conversely, if the light source is moving away from the observer, the observed frequency of light will be redshifted to lower frequencies. In Doppler OCT, the Doppler effect caused by moving particles in a sample is determined by measuring a shift in the phase between consecutive OCT interference fringe signals.

Figure A
Figure A

Figure B
Figure B

Fig. A shows the 3D surface reconstruction of the tadpole heart, while Fig. B demonstrates the complex blood flow pattern of the heart via a 3D color Doppler map.

Developmental Biology

Thorlabs’ Swept Source OCT Imaging System (OCS1300SS) with Doppler Imaging was used by researchers at the University of Toronto to study the cardiovascular system of living tadpoles. The series of images below show in vivo cross-sectional SS-OCT images of a beating tadpole heart superimposed with Doppler blood flow images. An optical Doppler cardiogram was obtained using a gated technique to increase the effective frame rate and improve the signal-to-noise ratio. The gating technique provides ultra high-speed visualization of the heart blood flow pattern in developing African frog embryos in both 3D and 4D (i.e., 3D + time) modes. This allows detailed visualization of the complex cardiac motion and hemodynamics in the beating heart.

Figure 1a
(1)
Figure 2
(2)
Figure 3
(3)
Figure 4
(4)
Figure 5
(5)
Figure 6
(6)

 

Reference:
A. Mariampillai, B.A. Standish, N.R. Munce, C. Randall, G. Liu, J.Y. Jiang, A.E. Cable, I.A. Vitkin, V.X.D. Yang, “Doppler optical cardiogram gated 2D color flow  maging at 1000 fps and 4D
in vivo visualization of embryonic heart at 45 fps on a swept source OCT system”, Optics Express15 , 1627 (2007).

Optical Coherence Tomography Tutorial

Optical Coherence Tomography (OCT) is a noninvasive optical imaging modality that provides real-time, 1D depth, 2D cross-sectional, and 3D volumetric images with micron-level resolution and millimeters of imaging depth. OCT images consist of structural information from a sample based on light backscattered from different layers of material within the sample. It can provide real-time imaging and is capable of being enhanced using birefringence contrast or functional blood flow imaging with optional extensions to the technology.

Thorlabs has designed a broad range of OCT imaging systems that cover several wavelengths, imaging resolutions, and speeds, while having a compact footprint for easy portability. Also, to increase our ability to provide OCT imaging systems that meet each customer’s unique requirements, we have designed a highly modular technology that can be optimized for varying applications.

Application Examples

Art Conservation
Art Conservation
Drug Coatings
Drug Coatings
3D Profiling
3D Profiling
In Vivo
In-vivo
Small Animal
Small Animal
Biology
Biology
Tissue Birefringence
Tissue Birefringence
Mouse Lung
Mouse Lung
Retina Cone Cells
Retina Cone Cells

OCT is the optical analog of ultrasound, with the tradeoff being lower imaging depth for significantly higher resolution (see Figure 1). With up to 15 mm imaging range and better than 5 micrometers in axial resolution, OCT fills a niche between ultrasound and confocal microscopy.

In addition to high resolution and greater imaging depth, the non-contract, noninvasive advantage of OCT makes it well suited for imaging samples such as biological tissue, small animals, and materials. Recent advances in OCT have led to a new class of technologies called Fourier Domain OCT, which has enabled high-speed imaging at rates greater than 700,000 lines per second.1 

Fourier Domain Optical Coherence Tomography (FD-OCT) is based on low-coherence interferometry, which utilizes the coherent properties of a light source to measure optical path length delays in a sample. In OCT, to obtain cross-sectional images with micron-level resolution, and interferometer is set up to measure optical path length differences between light reflected from the sample and reference arms.

There are two types of FD-OCT systems, each characterized by its light source and detection schemes: Spectral Domain OCT (SD-OCT) and Swept Source OCT (SS-OCT). In both types of systems, light is divided into sample and reference arms of an interferometer setup, as illustrated in Fig 2. SS-OCT uses coherent and narrowband light, whereas SD-OCT systems utilize broadband, low-coherence light sources. Back scattered light, attributed to variations in the index of refraction within a sample, is recoupled into the sample arm fiber and then combined with the light that has traveled a fixed optical path length along the reference arm. A resulting interferogram is measured through the detection arm of the interferometer.

The frequency of the interferogram measured by the sensor is related to depth locations of the reflectors in the sample. As a result, a depth reflectivity profile (A-scan) is produced by taking a Fourier transform of the detected interferogram. 2D cross-sectional images (B-scans) are produced by scanning the OCT sample beam across the sample. As the sample arm beam is scanned across the sample, a series of A-scans are collected to create the 2D image.

Similarly, when the OCT beam is scanned in a second direction, a series of 2D images are collected to produce a 3D volume data set. With FD-OCT, 2D images are collected on a time scale of milliseconds, and 3D images can be collected at rates now below 1 second. 

Spectral Domain OCT vs. Swept Source OCT

Spectral Domain and Swept Source OCT systems are based on the same fundamental principle but incorporate different technical approaches for producing the OCT interferogram. SD-OCT systems have no moving parts and therefore have high mechanical stability and low phase noise. Availability of a broad range of line cameras has also enabled development of SD-OCT systems with varying imaging speeds and sensitivities.

SS-OCT systems utilize a frequency swept light source and photodetector to rapidly generate the same type of interferogram. Due to the rapid sweeping of the swept laser source, high peak powers at each discrete wavelength can be used to illuminate the sample to provide greater sensitivity with little risk of optical damage.

FD-OCT Signal Processing

In Fourier Domain OCT, the interferogram is detected as a function of optical frequency. With a fixed optical delay in the reference arm, light reflected from different sample depths produces interference patterns with the different frequency components. A Fourier transform is used to resolve different depth reflections, thereby generating a depth profile of the sample (A-scan).

1V.Jayaraman, J. Jiang, H.Li, P. Heim, G. Cole, B. Potsaid, J. Fujimoto, and A. Cable, "OCT Imaging up to 760 kHz Axial Scan Rate Using Single-Mode 1310 nm MEMs-Tunable VCSELs with 100 nm Tuning Range," CLEO 2011 - Laser Applications to Photonic Applications, paper PDPB2 (2011).

Related Products

OCT Systems Comparison
Microscope Module
Swept Source OCT
Swept Source Lasers
Scan Lenses
OCT Balanced Detectors
OCT Interferometers
OCT Fiber Collimators
OCT Fiber Components

Please Give Us Your Feedback
 
Email   Feedback On
(Optional)
Contact Me:
Your email address will NOT be displayed.
 
 
Please type the following key into the field to submit this form:
Click Here if you can not read the security code.
This code is to prevent automated spamming of our site
Thank you for your understanding.
  
 
Would this product be useful to you?       Little Use    1 2 3 4 Very Useful

Enter Comments Below:
 
Characters remaining 8000    
Posted Comments:
Poster: jjurado
Posted Date: 2011-03-10 17:51:00.0
Response from Javier at Thorlabs to Scott Singleton: Thank you for your interest in our products. We will contact you shortly with more information about the Telesto OCT system. In the meantime, you can contact our Thorlabs UK office at techsupport.uk@thorlabs.com for further inquiries.
Poster: scott.singleton
Posted Date: 2011-03-10 20:24:31.0
Can you send me further technical details or a web address with richer information of the latest swept field OCT Telesto system. Also who is your UK represenatative. Scott Singleton Unilever Research Colworth Science Park MK45 7LT
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
TELESTO Support Documentation
TELESTO 1325 nm, Variable-Rate SD-OCT System
$70,000.00
Lead Time
Log In   |   My Account  |   Contact Us  |   Privacy Policy  |   Home  |   Site Index
Regional Websites: East Coast US US | Europe | Asia | China | Japan
Copyright 1999-2014 Thorlabs, Inc.
Sales: 1-973-579-7227
Technical Support: 1-973-300-3000


High Quality Thorlabs Logo 1000px: Save this Image